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Bharadwaj MS, Tyrrell DJ, Leng I, et al. Relationships between mitochondrial content and bioenergetics with obesity, body composition and fat distribution in healthy older adults. BMC Obes. 2015;2:40doi: 10.1186/s40608-015-0070-4.
Bharadwaj, M. S., Tyrrell, D. J., Leng, I., Demons, J. L., Lyles, M. F., Carr, J. J., Nicklas, B. J., & Molina, A. J. (2015). Relationships between mitochondrial content and bioenergetics with obesity, body composition and fat distribution in healthy older adults. BMC obesity, 240. https://doi.org/10.1186/s40608-015-0070-4
Bharadwaj, Manish S, et al. "Relationships between mitochondrial content and bioenergetics with obesity, body composition and fat distribution in healthy older adults." BMC obesity vol. 2 (2015): 40. doi: https://doi.org/10.1186/s40608-015-0070-4
Bharadwaj MS, Tyrrell DJ, Leng I, Demons JL, Lyles MF, Carr JJ, Nicklas BJ, Molina AJ. Relationships between mitochondrial content and bioenergetics with obesity, body composition and fat distribution in healthy older adults. BMC Obes. 2015 Oct 06;2:40. doi: 10.1186/s40608-015-0070-4. eCollection 2015. PMID: 26448868; PMCID: PMC4594906.
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BMC Obes. 2015 Oct 06;2:40. doi: 10.1186/s40608-015-0070-4. eCollection 2015.

Relationships between mitochondrial content and bioenergetics with obesity, body composition and fat distribution in healthy older adults.

BMC obesity

Manish S Bharadwaj, Daniel J Tyrrell, Iris Leng, Jamehl L Demons, Mary F Lyles, J Jeffrey Carr, Barbara J Nicklas, Anthony J A Molina

Affiliations

  1. Sticht Center on Aging & Department of Internal Medicine, Section on Gerontology and Geriatrics, Wake Forest School of Medicine, Winston-Salem, NC 27157 USA.
  2. Department of Biostatistical Sciences, Wake Forest School of Medicine, Winston-Salem, NC 27157 USA.
  3. Department of Radiology and Radiological Sciences, Vanderbilt University Medical Center, Nashville, TN 37203 USA.

PMID: 26448868 PMCID: PMC4594906 DOI: 10.1186/s40608-015-0070-4

Abstract

BACKGROUND: Mitochondrial function declines with age; however, the relationship between adiposity and mitochondrial function among older adults is unclear. This study examined relationships between skeletal muscle mitochondrial content and electron transport chain complex 2 driven respiration with whole body and thigh composition, body fat distribution, and insulin sensitivity in older adults.

METHODS: 25 healthy, sedentary, weight-stable men (N = 13) and women (N = 12) >65 years of age, with a BMI range of 18-35 kg/m(2), participated in this study. Vastus lateralis biopsies were analyzed for citrate synthase (CS) activity and succinate mediated respiration of isolated mitochondria. Whole body and thigh composition were measured by DXA and CT. HOMA-IR was calculated using fasting glucose and insulin as an estimate of insulin sensitivity.

RESULTS: Similar to reports in middle-aged adults, skeletal muscle CS activity was negatively correlated with BMI (R = -0.43) in our cohort of older adults. Higher total and thigh adiposity were correlated with lower CS activity independent of BMI (R = -0.50 and -0.71 respectively). Maximal complex 2 driven mitochondrial respiration was negatively correlated with lower body adiposity in males (R = -0.66). In this cohort of non-diabetic older adults, both HOMA-IR and insulin were positively correlated with CS activity when controlling for BMI (R = 0.57 and 0.66 respectively).

CONCLUSIONS: Adiposity and body composition are correlated with skeletal muscle mitochondrial content and electron transport chain function in healthy, sedentary, community dwelling, older adults. Specific relationships of mitochondrial bioenergetics with gender and insulin sensitivity are also apparent.

TRIAL REGISTRATION: ClinicalTrials.gov identifier NCT01049698.

Keywords: Adiposity; Aging; Mitochondria; Muscle; Obesity

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