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Zheng C, Ma Y, Wang X, et al. Functional analysis of the sporulation-specific diadenylate cyclase CdaS in Bacillus thuringiensis. Front Microbiol. 2015;6:908doi: 10.3389/fmicb.2015.00908.
Zheng, C., Ma, Y., Wang, X., Xie, Y., Ali, M. K., & He, J. (2015). Functional analysis of the sporulation-specific diadenylate cyclase CdaS in Bacillus thuringiensis. Frontiers in microbiology, 6908. https://doi.org/10.3389/fmicb.2015.00908
Zheng, Cao, et al. "Functional analysis of the sporulation-specific diadenylate cyclase CdaS in Bacillus thuringiensis." Frontiers in microbiology vol. 6 (2015): 908. doi: https://doi.org/10.3389/fmicb.2015.00908
Zheng C, Ma Y, Wang X, Xie Y, Ali MK, He J. Functional analysis of the sporulation-specific diadenylate cyclase CdaS in Bacillus thuringiensis. Front Microbiol. 2015 Sep 14;6:908. doi: 10.3389/fmicb.2015.00908. eCollection 2015. PMID: 26441857; PMCID: PMC4568413.
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Front Microbiol. 2015 Sep 14;6:908. doi: 10.3389/fmicb.2015.00908. eCollection 2015.

Functional analysis of the sporulation-specific diadenylate cyclase CdaS in Bacillus thuringiensis.

Frontiers in microbiology

Cao Zheng, Yang Ma, Xun Wang, Yuqun Xie, Maria K Ali, Jin He

Affiliations

  1. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University Wuhan, China.
  2. Key Laboratory of Fermentation Engineering (Ministry of Education), College of Bioengineering, Hubei University of Technology Wuhan, China.

PMID: 26441857 PMCID: PMC4568413 DOI: 10.3389/fmicb.2015.00908

Abstract

Cyclic di-AMP (c-di-AMP) is a recently discovered bacterial secondary messenger molecule, which is associated with various physiological functions. In the genus Bacillus, the intracellular level and turnover of c-di-AMP are mainly regulated by three diadenylate cyclases (DACs), including DisA, CdaA and CdaS, and two c-di-AMP-specific phosphodiesterases (GdpP and PgpH). In this study, we demonstrated that CdaS protein from B. thuringiensis is a hexameric DAC protein that can convert ATP or ADP to c-di-AMP in vitro and the N-terminal YojJ domain is essential for the DAC activity. Based on the markerless gene knock-out method, we demonstrated that the transcription of cdaS was initiated by the sporulation-specific sigma factor σ(H) and the deletion of cdaS significantly delayed sporulation and parasporal crystal formation. These findings contrast with similar experiments conducted using B. subtilis, wherein transcription of its cdaS was initiated by the sigma factor σ(G). Deletion of all the three DAC genes from a single strain was unsuccessful, suggesting that c-di-AMP is an indispensable molecule in B. thuringiensis. Phylogenetic analysis indicated increased diversity of CdaS in the B. cereus and B. subtilis Bacillus subgroups. In summary, this study identifies important aspects in the regulation of c-di-AMP in the genus Bacillus.

Keywords: Bacillus thuringiensis; CdaS; cyclic di-AMP; parasporal crystal; sporulation

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