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Dang WT, Xu D, Xie WG, et al. Expression of Caspase-1 Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Patients with Primary Gout in Different TCM Syndromes. Evid Based Complement Alternat Med. 2015;2015:361607doi: 10.1155/2015/361607.
Dang, W. T., Xu, D., Xie, W. G., & Zhou, J. G. (2015). Expression of Caspase-1 Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Patients with Primary Gout in Different TCM Syndromes. Evidence-based complementary and alternative medicine : eCAM, 2015361607. https://doi.org/10.1155/2015/361607
Dang, Wan-Tai, et al. "Expression of Caspase-1 Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Patients with Primary Gout in Different TCM Syndromes." Evidence-based complementary and alternative medicine : eCAM vol. 2015 (2015): 361607. doi: https://doi.org/10.1155/2015/361607
Dang WT, Xu D, Xie WG, Zhou JG. Expression of Caspase-1 Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Patients with Primary Gout in Different TCM Syndromes. Evid Based Complement Alternat Med. 2015;2015:361607. doi: 10.1155/2015/361607. Epub 2015 Oct 19. PMID: 26557856; PMCID: PMC4629022.
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Evid Based Complement Alternat Med. 2015;2015:361607. doi: 10.1155/2015/361607. Epub 2015 Oct 19.

Expression of Caspase-1 Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Patients with Primary Gout in Different TCM Syndromes.

Evidence-based complementary and alternative medicine : eCAM

Wan-Tai Dang, Dan Xu, Wen-Guang Xie, Jing-Guo Zhou

Affiliations

  1. School of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China ; Institute of Rheumatology and Immunology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China.
  2. Nephrology Department, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China.
  3. Institute of Rheumatology and Immunology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China.

PMID: 26557856 PMCID: PMC4629022 DOI: 10.1155/2015/361607

Abstract

A large number of studies have shown that cysteinyl aspartate specific protease-1 (CASP1) played an important role in the inflammatory response of primary gout, but the decreased expression of different CASP1 transcript variant could inhibit the activation of IL-1β. Our study mainly analyzed the expression level and function of CASP1 gene transcript variant mRNA in peripheral blood mononuclear cells of patients with gout in different TCM syndromes. The expression of CASP1 gene transcript variant and IL-1β mRNA in PBMCs were detected in patients with PG [acute phase (AP: 44 cases); nonacute phase (NAP: 52 cases)] and healthy controls (HC: 30 cases) by reverse transcription-polymerase chain reaction and/or real-time quantitative polymerase chain reaction. The expressions of plasma IL-1β in patients with PG and HC were detected by enzyme-linked immunosorbent assay. Dysregulated expression of the CASP1 gene and its transcript variant, plasma proinflammatory cytokines in all patients with primary gout in different TCM syndromes, correlation analysis showed that there was negative correlation between the expression of CASP1-gamma gene transcript variant mRNA and IL-1β protein in APPG group. The study suggested that CASP1 gene and its transcript variant may play a critical role in the inflammatory response of patients with PG in different phases and TCM syndromes.

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