Display options
Cite
Geng P, Wang S, Wang C, et al. Evaluation of the impact of Flos Daturae on rat hepatic cytochrome P450 enzymes by cocktail probe drugs. Int J Clin Exp Med. 2015;8(12):22310-8
Geng, P., Wang, S., Wang, C., Chen, J., Zhang, L., Yang, S., Wen, C., Zhou, Y., & Zhang, M. (2015). Evaluation of the impact of Flos Daturae on rat hepatic cytochrome P450 enzymes by cocktail probe drugs. International journal of clinical and experimental medicine, 8(12), 22310-8.
Geng, Peiwu, et al. "Evaluation of the impact of Flos Daturae on rat hepatic cytochrome P450 enzymes by cocktail probe drugs." International journal of clinical and experimental medicine vol. 8,12 (2015): 22310-8.
Geng P, Wang S, Wang C, Chen J, Zhang L, Yang S, Wen C, Zhou Y, Zhang M. Evaluation of the impact of Flos Daturae on rat hepatic cytochrome P450 enzymes by cocktail probe drugs. Int J Clin Exp Med. 2015 Dec 15;8(12):22310-8. eCollection 2015. PMID: 26885208; PMCID: PMC4729994.
Copy Download .nbib Format: NLM
Share it on

Int J Clin Exp Med. 2015 Dec 15;8(12):22310-8. eCollection 2015.

Evaluation of the impact of Flos Daturae on rat hepatic cytochrome P450 enzymes by cocktail probe drugs.

International journal of clinical and experimental medicine

Peiwu Geng, Shuanghu Wang, Chunjie Wang, Jianmiao Chen, Lijing Zhang, Suping Yang, Congcong Wen, Yunfang Zhou, Meiling Zhang

Affiliations

  1. The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui Lishui 323000, China.
  2. Special Procurement Ward, First Affiliated Hospital of Soochow University Suzhou 215006, China.
  3. Analytical and Testing Center of Wenzhou Medical University Wenzhou 325035, China.

PMID: 26885208 PMCID: PMC4729994

Abstract

Flos Daturae, known as "baimantuoluo" or "yangjinhua" in China, has been used for centuries in Traditional Chinese Medicine for the treatment of asthma, convulsions, pain, and rheumatism. To investigate the influences of Flos Daturae on the activities of rat CYP450 enzymes (CYP1A2, CYP2C9, CYP2C19, CYP2B6, CYP2D6 and CYP3A4) using cocktail probe drugs in vivo. A cocktail solution at a dose of 5 mL/kg, which contained phenacetin (10 mg/kg), tolbutamide (1 mg/kg), omeprazole (10 mg/kg), bupropion (10 mg/kg), metoprolol (10 mg/kg) and testosterone (10 mg/kg), was intragastric administered to rats treated with a single low or high dose of Flos Daturae decotion for 7days. Blood samples collected at a series of time-points in plasma were determined by UPLC-MS/MS. The corresponding pharmacokinetic parameters were calculated by the software of DAS 3.0. The results from the present in vivo study showed that Flos Daturae induce the activity of CYP2D6 enzyme with the decreased Cmax, AUC(0-∞) (P < 0.05) and the increased CL (P < 0.05). However, there were no significant differences of other probe drugs in plasma concentration and pharmacokinetic parameters. There were no significant effects on rat CYP1A2, CYP3A4, CYP2B6, CYP2C9 and CYP2C19 by Flos Daturae. Therefore, the resulting data suggested that caution was needed when Flos Daturae was co-administered with CYP2D6 substrates, which may result in treatment failure and herb-drug interactions.

Keywords: CYP450; Flos Daturae; UPLC-MS/MS; cocktail; rat

References

  1. Pharmazie. 2015 Jan;70(1):33-7 - PubMed
  2. Clin Pharmacokinet. 2009;48(12):761-804 - PubMed
  3. Biomed Chromatogr. 2015 Aug;29(8):1203-12 - PubMed
  4. J Ethnopharmacol. 2014 Sep 29;155(3):1473-82 - PubMed
  5. Clin Toxicol (Phila). 2010 Feb;48(2):104-10 - PubMed
  6. Pharmacol Ther. 2013 Apr;138(1):103-41 - PubMed
  7. Drug Metab Rev. 2002 Feb-May;34(1-2):83-448 - PubMed
  8. Annu Rev Pharmacol Toxicol. 2005;45:203-26 - PubMed
  9. Int J Mol Sci. 2013 Dec 10;14(12):24055-63 - PubMed
  10. Arch Pharm Res. 2008 Sep;31(9):1094-7 - PubMed
  11. Br J Clin Pharmacol. 2013 Mar;75(3):603-18 - PubMed
  12. Drug Metab Pharmacokinet. 2012;27(1):55-67 - PubMed
  13. Drug Metab Rev. 2009;41(2):89-295 - PubMed
  14. EXCLI J. 2014 Apr 02;13:347-91 - PubMed
  15. Biomed Res Int. 2014;2014:209393 - PubMed
  16. J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Oct 22;973C:68-75 - PubMed
  17. Br J Clin Pharmacol. 2009 Dec;68(6):928-35 - PubMed
  18. Pharmacogenomics J. 2014 Apr;14(2):107-14 - PubMed
  19. Biopharm Drug Dispos. 2014 May;35(4):228-36 - PubMed

Publication Types