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Yao J, Obara H, Sapkota A, et al. Development of three-dimensional integrated microchannel-electrode system to understand the particles' movement with electrokinetics. Biomicrofluidics. 2016;10(2):024105doi: 10.1063/1.4943859.
Yao, J., Obara, H., Sapkota, A., & Takei, M. (2016). Development of three-dimensional integrated microchannel-electrode system to understand the particles' movement with electrokinetics. Biomicrofluidics, 10(2), 024105. https://doi.org/10.1063/1.4943859
Yao, J, et al. "Development of three-dimensional integrated microchannel-electrode system to understand the particles' movement with electrokinetics." Biomicrofluidics vol. 10,2 (2016): 024105. doi: https://doi.org/10.1063/1.4943859
Yao J, Obara H, Sapkota A, Takei M. Development of three-dimensional integrated microchannel-electrode system to understand the particles' movement with electrokinetics. Biomicrofluidics. 2016 Mar 15;10(2):024105. doi: 10.1063/1.4943859. eCollection 2016 Mar. PMID: 27042247; PMCID: PMC4798993.
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Biomicrofluidics. 2016 Mar 15;10(2):024105. doi: 10.1063/1.4943859. eCollection 2016 Mar.

Development of three-dimensional integrated microchannel-electrode system to understand the particles' movement with electrokinetics.

Biomicrofluidics

J Yao, H Obara, A Sapkota, M Takei

Affiliations

  1. Department of Mechanical Engineering, Chiba University , Chiba 263-0022, Japan.
  2. Department of Mechanical Engineering, Tokyo Metropolitan University , Tokyo 192-0397, Japan.
  3. Department of Information and Computer Engineering, National Institute of Technology , Kisarazu College, Chiba 292-0041, Japan.

PMID: 27042247 PMCID: PMC4798993 DOI: 10.1063/1.4943859

Abstract

An optical transparent 3-D Integrated Microchannel-Electrode System (3-DIMES) has been developed to understand the particles' movement with electrokinetics in the microchannel. In this system, 40 multilayered electrodes are embedded at the 2 opposite sides along the 5 square cross-sections of the microchannel by using Micro Electro-Mechanical Systems technology in order to achieve the optical transparency at the other 2 opposite sides. The concept of the 3-DIMES is that the particles are driven by electrokinetic forces which are dielectrophoretic force, thermal buoyancy, electrothermal force, and electroosmotic force in a three-dimensional scope by selecting the excitation multilayered electrodes. As a first step to understand the particles' movement driven by electrokinetic forces in high conductive fluid (phosphate buffer saline (PBS)) with the 3-DIMES, the velocities of particles' movement with one pair of the electrodes are measured three dimensionally by Particle Image Velocimetry technique in PBS; meanwhile, low conductive fluid (deionized water) is used as a reference. Then, the particles' movement driven by the electrokinetic forces is discussed theoretically to estimate dominant forces exerting on the particles. Finally, from the theoretical estimation, the particles' movement mainly results from the dominant forces which are thermal buoyancy and electrothermal force, while the velocity vortex formed at the 2 edges of the electrodes is because of the electroosmotic force. The conclusions suggest that the 3-DIMES with PBS as high conductive fluid helps to understand the three-dimensional advantageous flow structures for cell manipulation in biomedical applications.

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