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Clark DR, Flynn KJ, Fabian H. Variation in elemental stoichiometry of the marine diatom Thalassiosira weissflogii (Bacillariophyceae) in response to combined nutrient stress and changes in carbonate chemistry. J Phycol. 2014;50(4):640-51doi: 10.1111/jpy.12208.
Clark, D. R., Flynn, K. J., & Fabian, H. (2014). Variation in elemental stoichiometry of the marine diatom Thalassiosira weissflogii (Bacillariophyceae) in response to combined nutrient stress and changes in carbonate chemistry. Journal of phycology, 50(4), 640-51. https://doi.org/10.1111/jpy.12208
Clark, Darren R, et al. "Variation in elemental stoichiometry of the marine diatom Thalassiosira weissflogii (Bacillariophyceae) in response to combined nutrient stress and changes in carbonate chemistry." Journal of phycology vol. 50,4 (2014): 640-51. doi: https://doi.org/10.1111/jpy.12208
Clark DR, Flynn KJ, Fabian H. Variation in elemental stoichiometry of the marine diatom Thalassiosira weissflogii (Bacillariophyceae) in response to combined nutrient stress and changes in carbonate chemistry. J Phycol. 2014 Aug;50(4):640-51. doi: 10.1111/jpy.12208. Epub 2014 Jul 10. PMID: 26988448.
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J Phycol. 2014 Aug;50(4):640-51. doi: 10.1111/jpy.12208. Epub 2014 Jul 10.

Variation in elemental stoichiometry of the marine diatom Thalassiosira weissflogii (Bacillariophyceae) in response to combined nutrient stress and changes in carbonate chemistry.

Journal of phycology

Darren R Clark, Kevin J Flynn, Heiner Fabian

Affiliations

  1. Plymouth Marine Laboratory, Prospect Place, Plymouth, PL1 3DH, UK.
  2. Centre for Sustainable Aquatic Research (CSAR), Swansea University, Wallace Building, Swansea, SA2 8PP, UK.

PMID: 26988448 DOI: 10.1111/jpy.12208

Abstract

The combined consequences of the multi-stressors of pH and nutrient availability upon the growth of a marine diatom were investigated. Thalassiosira weissflogii was grown in N- or P-limited batch culture in sealed systems, with pH commencing at 8.2 ("extant" conditions) or 7.6 ("ocean acidification" [OA] conditions), and then pH was allowed to either drift with growth, or was held fixed. Results indicated that within the pH range tested, the stability of environmental pH rather than its value (i.e., OA vs. extant) fundamentally influenced biomass accumul-ation and C:N:P stoichiometry. Despite large changes in total alkalinity in the fixed pH systems, final biomass production was consistently greater in these systems than that in drifting pH systems. In drift systems, pH increased to exceed pH 9.5, a level of alkalinity that was inhibitory to growth. No statis-tically significant differences between pH treatments were measured for N:C, P:C or N:P ratios during nutrient-replete growth, although the diatom expre-ssed greater plasticity in P:C and N:P ratios than in N:C during this growth phase. During nutrient-deplete conditions, the capacity for uncoupled carbon fixa-tion at fixed pH was considerably greater than that measured in drift pH systems, leading to strong contrasts in C:N:P stoichiometry between these treatments. Whether environmental pH was stable or drifted directly influenced the extent of physiological stress. In contrast, few distinctions could be drawn between "extant" versus "OA" conditions for cell physiology.

© 2014 The Authors. Journal of Phycology published by Wiley Periodicals, Inc. on behalf of Phycological Society of America.

Keywords: acclimation; alkalinity; diatom; growth rate; nutrient; pH

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