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Hagisawa K, Saito A, Kinoshita M, et al. Effective control of massive venous bleeding by "multioverlapping therapy" using polysaccharide nanosheets in a rabbit inferior vena cava injury model. J Vasc Surg Venous Lymphat Disord. 2013;1(3):289-97doi: 10.1016/j.jvsv.2013.04.001.
Hagisawa, K., Saito, A., Kinoshita, M., Fujie, T., Otani, N., Shono, S., Park, Y. K., & Takeoka, S. (2013). Effective control of massive venous bleeding by "multioverlapping therapy" using polysaccharide nanosheets in a rabbit inferior vena cava injury model. Journal of vascular surgery. Venous and lymphatic disorders, 1(3), 289-97. https://doi.org/10.1016/j.jvsv.2013.04.001
Hagisawa, Kohsuke, et al. "Effective control of massive venous bleeding by "multioverlapping therapy" using polysaccharide nanosheets in a rabbit inferior vena cava injury model." Journal of vascular surgery. Venous and lymphatic disorders vol. 1,3 (2013): 289-97. doi: https://doi.org/10.1016/j.jvsv.2013.04.001
Hagisawa K, Saito A, Kinoshita M, Fujie T, Otani N, Shono S, Park YK, Takeoka S. Effective control of massive venous bleeding by "multioverlapping therapy" using polysaccharide nanosheets in a rabbit inferior vena cava injury model. J Vasc Surg Venous Lymphat Disord. 2013 Jul;1(3):289-97. doi: 10.1016/j.jvsv.2013.04.001. Epub 2013 May 18. PMID: 26992589.
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J Vasc Surg Venous Lymphat Disord. 2013 Jul;1(3):289-97. doi: 10.1016/j.jvsv.2013.04.001. Epub 2013 May 18.

Effective control of massive venous bleeding by "multioverlapping therapy" using polysaccharide nanosheets in a rabbit inferior vena cava injury model.

Journal of vascular surgery. Venous and lymphatic disorders

Kohsuke Hagisawa, Akihiro Saito, Manabu Kinoshita, Toshinori Fujie, Naoki Otani, Satoshi Shono, Young-Kwang Park, Shinji Takeoka

Affiliations

  1. Department of Physiology, National Defense Medical College, Tokorozawa, Saitama, Japan.
  2. Graduate School of Advanced Science and Engineering, Department of Life Science and Medical Bioscience, Waseda University, Shinjuku, Tokyo, Japan.
  3. Department of Immunology and Microbiology, National Defense Medical College, Tokorozawa, Saitama, Japan. Electronic address: [email protected].
  4. Department of Neurosurgery, National Defense Medical College, Tokorozawa, Saitama, Japan.
  5. Department of Immunology and Microbiology, National Defense Medical College, Tokorozawa, Saitama, Japan.
  6. Graduate School of Science and Engineering, Department of Bioscience and Bioengineering, Waseda University, Shinjuku, Tokyo, Japan.

PMID: 26992589 DOI: 10.1016/j.jvsv.2013.04.001

Abstract

OBJECTIVE: To investigate the efficacy of multioverlapping therapy using a polysaccharide nanosheet having 75-nm thickness for sealing and stopping massive venous hemorrhage.

METHODS: The hydrostatic durability of the polysaccharide nanosheet was evaluated in vitro when secured to an incised silicon tube. For in vivo studies, the inferior vena cava (IVC) of rabbits was cut longitudinally, and multiple polysaccharide nanosheets were overlapped onto the injured IVC.

RESULTS: The mechanical hydrostatic durability of the nanosheets was gradually augmented by an increasing number of multilayered nanosheets in vitro. This durability was saturated at 80 ± 6 mm Hg by four layers of nanosheets, which was robust enough to seal injured vessel walls of the large IVC. Multioverlapping therapy using nanosheets effectively sealed and stopped bleeding from the injured IVC in vivo. One month later, no inflammatory tissue response was observed around the nanosheet attachment sites of the IVC, while conventional suturing repair in control rabbits showed a severe inflammatory response around the sutured area.

CONCLUSIONS: The multioverlapping therapy using the polysaccharide nanosheets will effectively stop massive venous bleeding without adverse effects in the immediate or chronic postoperative setting.

Copyright © 2013 Society for Vascular Surgery. Published by Elsevier Inc. All rights reserved.

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