Display options
Cite
Huang JH, Han D, Ruggles ME, et al. Characterization of enzymatic micromachining for construction of variable cross-section microchannel topologies. Biomicrofluidics. 2016;10(3):033102doi: 10.1063/1.4948508.
Huang, J. H., Han, D., Ruggles, M. E., Jayaraman, A., & Ugaz, V. M. (2016). Characterization of enzymatic micromachining for construction of variable cross-section microchannel topologies. Biomicrofluidics, 10(3), 033102. https://doi.org/10.1063/1.4948508
Huang, Jen-Huang, et al. "Characterization of enzymatic micromachining for construction of variable cross-section microchannel topologies." Biomicrofluidics vol. 10,3 (2016): 033102. doi: https://doi.org/10.1063/1.4948508
Huang JH, Han D, Ruggles ME, Jayaraman A, Ugaz VM. Characterization of enzymatic micromachining for construction of variable cross-section microchannel topologies. Biomicrofluidics. 2016 May 03;10(3):033102. doi: 10.1063/1.4948508. eCollection 2016 May. PMID: 27190566; PMCID: PMC4859826.
Copy Download .nbib Format: NLM
Share it on

Biomicrofluidics. 2016 May 03;10(3):033102. doi: 10.1063/1.4948508. eCollection 2016 May.

Characterization of enzymatic micromachining for construction of variable cross-section microchannel topologies.

Biomicrofluidics

Jen-Huang Huang, Duanduan Han, Molly E Ruggles, Arul Jayaraman, Victor M Ugaz

Affiliations

  1. Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843-3122, USA.

PMID: 27190566 PMCID: PMC4859826 DOI: 10.1063/1.4948508

Abstract

The ability to harness enzymatic activity as an etchant to precisely machine biodegradable substrates introduces new possibilities for microfabrication. This flow-based etching is straightforward to implement, enabling patterning of microchannels with topologies that incorporate variable depth along the cross-sectional dimension. Additionally, unlike conventional small-molecule formulations, the macromolecular nature of enzymatic etchants enables features to be precisely positioned. Here, we introduce a kinetic model to characterize the enzymatic machining process and its localization by co-injection of a macromolecular inhibitor species. Our model captures the interaction between enzyme, inhibitor, and substrate under laminar flow, enabling rational prediction of etched microchannel profiles so that cross-sectional topologies incorporating complex lateral variations in depth can be constructed. We also apply this approach to achieve simultaneous widening of an entire network of microchannels produced in the biodegradable polymeric substrate poly(lactic acid), laying a foundation to construct systems incorporating a broad range of internal cross-sectional dimensions by manipulating the process conditions.

References

  1. Nat Methods. 2005 Aug;2(8):599-605 - PubMed
  2. Biomaterials. 1996 Jan;17(2):103-14 - PubMed
  3. Analyst. 2015 Jan 7;140(1):22-38 - PubMed
  4. Anal Chem. 1998 Dec 1;70(23):4974-84 - PubMed
  5. Anal Chem. 2009 May 1;81(9):3471-7 - PubMed
  6. Nature. 2014 Mar 13;507(7491):181-9 - PubMed
  7. Lab Chip. 2009 Jul 21;9(14):1994-6 - PubMed
  8. PLoS One. 2013 Sep 02;8(9):e73188 - PubMed
  9. Anal Chem. 2005 Nov 15;77(22):7478-82 - PubMed
  10. Angew Chem Int Ed Engl. 2012 Sep 17;51(38):9619-23 - PubMed
  11. Nat Rev Drug Discov. 2006 Mar;5(3):210-8 - PubMed
  12. J Biol Chem. 2003 Dec 5;278(49):48735-44 - PubMed
  13. Biomaterials. 2014 Aug;35(26):7308-25 - PubMed

Publication Types