Display options
Cite
Jones J, Jones M, Plate C, et al. Liquid Chromatography-Tandem Mass Spectrometry Assay to Detect Ethyl Glucuronide in Human Fingernail: Comparison to Hair and Gender Differences. Am J Analyt Chem. 2012;3(1):83-91doi: 10.4236/ajac.2012.31012.
Jones, J., Jones, M., Plate, C., Lewis, D., Fendrich, M., Berger, L., & Fuhrmann, D. (2012). Liquid Chromatography-Tandem Mass Spectrometry Assay to Detect Ethyl Glucuronide in Human Fingernail: Comparison to Hair and Gender Differences. American journal of analytical chemistry, 3(1), 83-91. https://doi.org/10.4236/ajac.2012.31012
Jones, Joseph, et al. "Liquid Chromatography-Tandem Mass Spectrometry Assay to Detect Ethyl Glucuronide in Human Fingernail: Comparison to Hair and Gender Differences." American journal of analytical chemistry vol. 3,1 (2012): 83-91. doi: https://doi.org/10.4236/ajac.2012.31012
Jones J, Jones M, Plate C, Lewis D, Fendrich M, Berger L, Fuhrmann D. Liquid Chromatography-Tandem Mass Spectrometry Assay to Detect Ethyl Glucuronide in Human Fingernail: Comparison to Hair and Gender Differences. Am J Analyt Chem. 2012 Jan;3(1):83-91. doi: 10.4236/ajac.2012.31012. Epub 2012 Jan 01. PMID: 27134762; PMCID: PMC4847958.
Copy Download .nbib Format: NLM
Share it on

Am J Analyt Chem. 2012 Jan;3(1):83-91. doi: 10.4236/ajac.2012.31012. Epub 2012 Jan 01.

Liquid Chromatography-Tandem Mass Spectrometry Assay to Detect Ethyl Glucuronide in Human Fingernail: Comparison to Hair and Gender Differences.

American journal of analytical chemistry

Joseph Jones, Mary Jones, Charles Plate, Douglas Lewis, Michael Fendrich, Lisa Berger, Daniel Fuhrmann

Affiliations

  1. United States Drug Testing Laboratories, Inc., Des Plaines, USA.
  2. Center for Addiction and Behavioral Health Research, University of Wisconsin-Milwaukee, Milwaukee, USA.

PMID: 27134762 PMCID: PMC4847958 DOI: 10.4236/ajac.2012.31012

Abstract

Over the past decade, the use of hair specimens for the long-term detection of the alcohol biomarker ethyl glucuronide has been increasing in popularity and usage. We evaluated the usefulness of fingernail clippings as a suitable alternative to hair for ethyl glucuronide detection. A liquid chromatography-tandem mass spectrometry method for the detection of ethyl glucuronide in fingernail clippings was fully validated and used to analyze the hair and/or fingernail specimens of 606 college-aged study participants. The limit of detection was 2 pg/mg, the limit of quantitation was 8 pg/mg and the method was linear from 8 to 2000 pg/mg. Intra- and inter-assay imprecision studies at three different concentrations (20, 40, 200 pg/mg) were all within 7.8% and all intra- and inter-assay bias studies at these levels were within 115.1% of target concentration. Ethyl glucuronide levels in fingernail (mean = 29.1 ± 55.6 pg/mg) were higher than ethyl glucuronide levels in hair (mean = 9.48 ± 22.3 pg/mg) and a correlation of the matched pairs was observed (

Keywords: Alcohol; Biomarkers; Ethyl Glucuronide; Fingernail; Hair; LC-MS/MS; Liquid Chromatography-Tandem Mass Spectrometry

References

  1. Addiction. 2010 Feb;105(2):226-39 - PubMed
  2. J Anal Toxicol. 2009 May;33(4):204-7 - PubMed
  3. Forensic Sci Int. 2012 May 10;218(1-3):123-5 - PubMed
  4. Forensic Sci Int. 2010 May 20;198(1-3):23-7 - PubMed
  5. Alcohol Alcohol. 1999 Jan-Feb;34(1):71-7 - PubMed
  6. J Anal Toxicol. 1999 May-Jun;23(3):147-52 - PubMed
  7. Clin Chem. 2007 Oct;53(10):1855-7 - PubMed
  8. Forensic Sci Int. 2003 Jan 28;131(2-3):90-7 - PubMed
  9. Forensic Sci Int. 2006 Jun 2;159(2-3):218-22 - PubMed
  10. J Anal Toxicol. 2006 Oct;30(8):643-4 - PubMed
  11. Clin Pharmacokinet. 2000 Feb;38(2):95-110 - PubMed
  12. Anal Bioanal Chem. 2011 Apr;400(1):183-8 - PubMed
  13. Forensic Sci Int. 2004 Oct 29;145(2-3):167-73 - PubMed
  14. J Anal Toxicol. 2004 Mar;28(2):128-31 - PubMed
  15. Alcohol Clin Exp Res. 2010 Jun;34(6):955-67 - PubMed
  16. Forensic Sci Int. 2012 Feb 10;215(1-3):51-5 - PubMed
  17. Lancet. 1973 Jun 9;1(7815):1267-71 - PubMed
  18. Am J Psychiatry. 1971 Jun;127(12):1653-8 - PubMed
  19. Anal Chem. 2003 Jul 1;75(13):3019-30 - PubMed
  20. Forensic Sci Int. 2004 Oct 29;145(2-3):161-6 - PubMed
  21. Forensic Sci Int. 2002 Aug 14;128(1-2):59-65 - PubMed
  22. Addiction. 2006 Oct;101(10):1408-12 - PubMed
  23. Alcohol Alcohol. 2002 Jan-Feb;37(1):61-6 - PubMed
  24. J Anal Toxicol. 2008 Mar;32(2):156-9 - PubMed
  25. J Mass Spectrom. 2006 Jan;41(1):34-42 - PubMed
  26. Ther Drug Monit. 1997 Aug;19(4):386-9 - PubMed
  27. Alcohol Alcohol. 2004 Sep-Oct;39(5):445-9 - PubMed
  28. Alcohol Alcohol. 2000 May-Jun;35(3):283-5 - PubMed
  29. Forensic Sci Int. 2012 May 10;218(1-3):97-100 - PubMed
  30. J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Jan 18;830(2):293-300 - PubMed
  31. Forensic Sci Int. 2010 Mar 20;196(1-3):101-10 - PubMed

Publication Types

Grant support