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Mato EPM, Pokam-Fosso PE, Atogho-Tiedeu B, et al. The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population. BMC Obes. 2016;3:26doi: 10.1186/s40608-016-0104-6.
Mato, E. P. M., Pokam-Fosso, P. E., Atogho-Tiedeu, B., Noubiap, J. J. N., Evehe, M. S., Djokam-Dadjeu, R., Donfack, O. S., Ngwa, E. N., Guewo-Fokeng, M., Mbacham, W. F., Sobngwi, E., & Mbanya, J. C. (2016). The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population. BMC obesity, 326. https://doi.org/10.1186/s40608-016-0104-6
Mato, Edith Pascale M, et al. "The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population." BMC obesity vol. 3 (2016): 26. doi: https://doi.org/10.1186/s40608-016-0104-6
Mato EPM, Pokam-Fosso PE, Atogho-Tiedeu B, Noubiap JJN, Evehe MS, Djokam-Dadjeu R, Donfack OS, Ngwa EN, Guewo-Fokeng M, Mbacham WF, Sobngwi E, Mbanya JC. The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population. BMC Obes. 2016 May 12;3:26. doi: 10.1186/s40608-016-0104-6. eCollection 2016. PMID: 27239321; PMCID: PMC4866427.
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BMC Obes. 2016 May 12;3:26. doi: 10.1186/s40608-016-0104-6. eCollection 2016.

The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population.

BMC obesity

Edith Pascale M Mato, Priscille Eunice Pokam-Fosso, Barbara Atogho-Tiedeu, Jean Jacques N Noubiap, Marie-Solange Evehe, Rosine Djokam-Dadjeu, Olivier Sontsa Donfack, Elvis Ndonwi Ngwa, Magellan Guewo-Fokeng, Wilfred F Mbacham, Eugene Sobngwi, Jean Claude Mbanya

Affiliations

  1. Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.
  2. Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
  3. Department of Medicine, Groote Schuur Hospital and University of Cape Town, Cape Town, South Africa.
  4. Medical Diagnostic Center, Yaoundé, Cameroon.
  5. Laboratory for Public Health Research and Biotechnologies, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
  6. Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.
  7. National Obesity Center, Yaoundé Central Hospital and Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon.

PMID: 27239321 PMCID: PMC4866427 DOI: 10.1186/s40608-016-0104-6

Abstract

BACKGROUND: Peroxisome proliferator-activated receptor gamma 2 (PPAR-γ2) is a transcription factor with a key role in adipocyte differentiation, lipid storage and glucose homeostasis. The Ala allele of the common Pro12Ala polymorphism in the isoform PPAR-γ2 is at the center of many controversies because in some populations, it has been observed to be associated with T2DM or obesity but, not in others. The aim of this study was to investigate the association of Pro12Ala polymorphism in the PPAR-γ2 gene with susceptibility to obesity or T2DM in a Cameroonian population.

METHODS: This case-control study included 62 obese, 60 T2DM patients and 120 controls (60 non obese and 60 patients without T2DM), all unrelated and of Cameroonian origin. PPAR-γ2 was examined by genotyping for Pro12Ala using the Restriction Fragment Length Polymorphism - Polymerase Chain Reaction (PCR - RFLP).

RESULTS: A portion of the 270 base pair bands of the PPAR-γ2 gene was successfully amplified. The Ala12 variant was totally absent from the study population, all participants being homozygote Pro/Pro.

CONCLUSION: PPAR-γ2 Pro12Ala gene polymorphism may not be associated with obesity and T2DM. These results suggest that, PPAR-γ2 is unlikely a major gene for obesity or T2DM in the study population.

Keywords: Genetic association; Obesity; PPAR gamma2; PPAR-γ2; Pro12Ala; Type 2 diabetes mellitus

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