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Aoki H, Nishioka K, Mankura M, et al. The Incorporation and Accumulation of Supplemented Fatty Acids in Mucor hiemalis HA-30. Biosci Biotechnol Biochem. 1999;63(6):1032-6doi: 10.1271/bbb.63.1032.
Aoki, H., Nishioka, K., Mankura, M., Endo, Y., & Fujimoto, K. (1999). The Incorporation and Accumulation of Supplemented Fatty Acids in Mucor hiemalis HA-30. Bioscience, biotechnology, and biochemistry, 63(6), 1032-6. https://doi.org/10.1271/bbb.63.1032
Aoki, H, et al. "The Incorporation and Accumulation of Supplemented Fatty Acids in Mucor hiemalis HA-30." Bioscience, biotechnology, and biochemistry vol. 63,6 (1999): 1032-6. doi: https://doi.org/10.1271/bbb.63.1032
Aoki H, Nishioka K, Mankura M, Endo Y, Fujimoto K. The Incorporation and Accumulation of Supplemented Fatty Acids in Mucor hiemalis HA-30. Biosci Biotechnol Biochem. 1999;63(6):1032-6. doi: 10.1271/bbb.63.1032. PMID: 27389336.
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Biosci Biotechnol Biochem. 1999;63(6):1032-6. doi: 10.1271/bbb.63.1032.

The Incorporation and Accumulation of Supplemented Fatty Acids in Mucor hiemalis HA-30.

Bioscience, biotechnology, and biochemistry

H Aoki, K Nishioka, M Mankura, Y Endo, K Fujimoto

Affiliations

  1. a Research Laboratory, Ikeda Food Research Co., Ltd.
  2. b Department of Science of Biological Function, Graduate School of Agricultural Science, Tohoku University.

PMID: 27389336 DOI: 10.1271/bbb.63.1032

Abstract

Accumulation of free unsaturated fatty acids, added individually to the medium, into cellular triacylglycerol (TG) were examined using a fungus, Mucor hiemalis HA-30, which could produce the eicosapentaenoic acid (20:5n-3)-enriched TG. Linoleic (18:2n-6), γ-linolenic (18:3n-6), arachidonic acids (20:4n-6), and 20:5n-3 were favored for accumulation in TG and each fatty acid accumulated in TG at about 80% purity. However, docosahexaenoic acid (22:6n-3) was not efficiently accumulated in TG and its content was about 60%. Incorporation and accumulation of various fatty acids added to the cultivation medium were investigated using a model fatty acids mixture. Generally, unsaturated fatty acids were efficiently incorporated into cells more than saturated fatty acids. Fatty acids with odd-numbered double bonds such as 18:3n-3, 18:3n-6, and 20:5n-3 showed the higher incorporation efficiency compared with those with even-numbered double bonds such as 18:2n-6, 20:4n-6, and 22:6n-3. On the other hand, saturated and monounsaturated fatty acids of 16:0, 18:0, and 18:1 were preferentially accumulated in microbial cells over other fatty acids.

Keywords: Mucor; accumulation; fatty acid; incorporation

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