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Mayourian J, Savizky RM, Sobie EA, et al. Modeling Electrophysiological Coupling and Fusion between Human Mesenchymal Stem Cells and Cardiomyocytes. PLoS Comput Biol. 2016;12(7):e1005014doi: 10.1371/journal.pcbi.1005014.
Mayourian, J., Savizky, R. M., Sobie, E. A., & Costa, K. D. (2016). Modeling Electrophysiological Coupling and Fusion between Human Mesenchymal Stem Cells and Cardiomyocytes. PLoS computational biology, 12(7), e1005014. https://doi.org/10.1371/journal.pcbi.1005014
Mayourian, Joshua, et al. "Modeling Electrophysiological Coupling and Fusion between Human Mesenchymal Stem Cells and Cardiomyocytes." PLoS computational biology vol. 12,7 (2016): e1005014. doi: https://doi.org/10.1371/journal.pcbi.1005014
Mayourian J, Savizky RM, Sobie EA, Costa KD. Modeling Electrophysiological Coupling and Fusion between Human Mesenchymal Stem Cells and Cardiomyocytes. PLoS Comput Biol. 2016 Jul 25;12(7):e1005014. doi: 10.1371/journal.pcbi.1005014. eCollection 2016 Jul. PMID: 27454812; PMCID: PMC4959759.
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PLoS Comput Biol. 2016 Jul 25;12(7):e1005014. doi: 10.1371/journal.pcbi.1005014. eCollection 2016 Jul.

Modeling Electrophysiological Coupling and Fusion between Human Mesenchymal Stem Cells and Cardiomyocytes.

PLoS computational biology

Joshua Mayourian, Ruben M Savizky, Eric A Sobie, Kevin D Costa

Affiliations

  1. Cardiovascular Research Center, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America.
  2. Department of Chemistry, The Cooper Union, New York, New York, United States of America.
  3. Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai, New York, New York, United States of America.

PMID: 27454812 PMCID: PMC4959759 DOI: 10.1371/journal.pcbi.1005014

Abstract

Human mesenchymal stem cell (hMSC) delivery has demonstrated promise in preclinical and clinical trials for myocardial infarction therapy; however, broad acceptance is hindered by limited understanding of hMSC-human cardiomyocyte (hCM) interactions. To better understand the electrophysiological consequences of direct heterocellular connections between hMSCs and hCMs, three original mathematical models were developed, representing an experimentally verified triad of hMSC families with distinct functional ion channel currents. The arrhythmogenic risk of such direct electrical interactions in the setting of healthy adult myocardium was predicted by coupling and fusing these hMSC models to the published ten Tusscher midcardial hCM model. Substantial variations in action potential waveform-such as decreased action potential duration (APD) and plateau height-were found when hCMs were coupled to the two hMSC models expressing functional delayed rectifier-like human ether à-go-go K+ channel 1 (hEAG1); the effects were exacerbated for fused hMSC-hCM hybrid cells. The third family of hMSCs (Type C), absent of hEAG1 activity, led to smaller single-cell action potential alterations during coupling and fusion, translating to longer tissue-level mean action potential wavelength. In a simulated 2-D monolayer of cardiac tissue, re-entry vulnerability with low (5%) hMSC insertion was approximately eight-fold lower with Type C hMSCs compared to hEAG1-functional hMSCs. A 20% decrease in APD dispersion by Type C hMSCs compared to hEAG1-active hMSCs supports the claim of reduced arrhythmogenic potential of this cell type with low hMSC insertion. However, at moderate (15%) and high (25%) hMSC insertion, the vulnerable window increased independent of hMSC type. In summary, this study provides novel electrophysiological models of hMSCs, predicts possible arrhythmogenic effects of hMSCs when directly coupled to healthy hCMs, and proposes that isolating a subset of hMSCs absent of hEAG1 activity may offer increased safety as a cell delivery cardiotherapy at low levels of hMSC-hCM coupling.

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