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Tu H, Liu Y, Turchinovich D, et al. Stain-free histopathology by programmable supercontinuum pulses. Nat Photonics. 2016;10(8):534-540doi: 10.1038/nphoton.2016.94.
Tu, H., Liu, Y., Turchinovich, D., Marjanovic, M., Lyngsø, J., Lægsgaard, J., Chaney, E. J., Zhao, Y., You, S., Wilson, W. L., Xu, B., Dantus, M., & Boppart, S. A. (2016). Stain-free histopathology by programmable supercontinuum pulses. Nature photonics, 10(8), 534-540. https://doi.org/10.1038/nphoton.2016.94
Tu, Haohua, et al. "Stain-free histopathology by programmable supercontinuum pulses." Nature photonics vol. 10,8 (2016): 534-540. doi: https://doi.org/10.1038/nphoton.2016.94
Tu H, Liu Y, Turchinovich D, Marjanovic M, Lyngsø J, Lægsgaard J, Chaney EJ, Zhao Y, You S, Wilson WL, Xu B, Dantus M, Boppart SA. Stain-free histopathology by programmable supercontinuum pulses. Nat Photonics. 2016 Aug;10(8):534-540. doi: 10.1038/nphoton.2016.94. Epub 2016 May 23. PMID: 27668009; PMCID: PMC5031149.
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Nat Photonics. 2016 Aug;10(8):534-540. doi: 10.1038/nphoton.2016.94. Epub 2016 May 23.

Stain-free histopathology by programmable supercontinuum pulses.

Nature photonics

Haohua Tu, Yuan Liu, Dmitry Turchinovich, Marina Marjanovic, Jens Lyngsø, Jesper Lægsgaard, Eric J Chaney, Youbo Zhao, Sixian You, William L Wilson, Bingwei Xu, Marcos Dantus, Stephen A Boppart

Affiliations

  1. Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
  2. Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Max Planck Institute for Polymer Research, Ackermannweg 10, Mainz, 55128, Germany.
  3. Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Max Planck Institute for Polymer Research, Ackermannweg 10, Mainz, 55128, Germany.
  4. NKT Photonics A/S, Blokken 84, 3460 Birkerød, Denmark.
  5. DTU Fotonik, Technical University of Denmark, Ørsteds Plads 343, 2800 Lyngby, Denmark.
  6. Department of Materials Science and Engineering, Frederick Seitz Materials Research Laboratory, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
  7. Biophotonic Solutions Inc., East Lansing, Michigan 48823, USA.
  8. Department of Chemistry and Department of Physics, Michigan State University, East Lansing, Michigan 48824, USA.
  9. Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, Max Planck Institute for Polymer Research, Ackermannweg 10, Mainz, 55128, Germany; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; College of Medicine, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

PMID: 27668009 PMCID: PMC5031149 DOI: 10.1038/nphoton.2016.94

Abstract

The preparation, staining, visualization, and interpretation of histological images of tissue is well-accepted as the gold standard process for the diagnosis of disease. These methods were developed historically, and are used ubiquitously in pathology, despite being highly time and labor intensive. Here we introduce a unique optical imaging platform and methodology for label-free multimodal multiphoton microscopy that uses a novel photonic crystal fiber source to generate tailored chemical contrast based on programmable supercontinuum pulses. We demonstrate collection of optical signatures of the tumor microenvironment, including evidence of mesoscopic biological organization, tumor cell migration, and (lymph-)angiogenesis collected directly from fresh

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