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Logan C, Todorof K, Fiorillo SP, et al. Rapid Multiplexed Immunoassay for Detection of Antibodies to Kaposi's Sarcoma-Associated Herpesvirus. PLoS One. 2016;11(9):e0163616doi: 10.1371/journal.pone.0163616.
Logan, C., Todorof, K., Fiorillo, S. P., Campbell, T. B., Elder, J. H., Borok, M., Gudza, I., Gwanzura, L., Ndemera, B., Lochhead, M. J., Benson, C. A., & Schooley, R. T. (2016). Rapid Multiplexed Immunoassay for Detection of Antibodies to Kaposi's Sarcoma-Associated Herpesvirus. PloS one, 11(9), e0163616. https://doi.org/10.1371/journal.pone.0163616
Logan, Cathy, et al. "Rapid Multiplexed Immunoassay for Detection of Antibodies to Kaposi's Sarcoma-Associated Herpesvirus." PloS one vol. 11,9 (2016): e0163616. doi: https://doi.org/10.1371/journal.pone.0163616
Logan C, Todorof K, Fiorillo SP, Campbell TB, Elder JH, Borok M, Gudza I, Gwanzura L, Ndemera B, Lochhead MJ, Benson CA, Schooley RT. Rapid Multiplexed Immunoassay for Detection of Antibodies to Kaposi's Sarcoma-Associated Herpesvirus. PLoS One. 2016 Sep 26;11(9):e0163616. doi: 10.1371/journal.pone.0163616. eCollection 2016. PMID: 27669509; PMCID: PMC5036886.
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PLoS One. 2016 Sep 26;11(9):e0163616. doi: 10.1371/journal.pone.0163616. eCollection 2016.

Rapid Multiplexed Immunoassay for Detection of Antibodies to Kaposi's Sarcoma-Associated Herpesvirus.

PloS one

Cathy Logan, Kathryn Todorof, Suzanne P Fiorillo, Thomas B Campbell, John H Elder, Margaret Borok, Ivy Gudza, Lovemore Gwanzura, Buxton Ndemera, Michael J Lochhead, Constance A Benson, Robert T Schooley

Affiliations

  1. Department of Medicine, Division of Infectious Diseases, University of California San Diego, San Diego, CA, United States of America.
  2. MBio Diagnostics, Inc., Boulder, CO, United States of America.
  3. Department of Medicine, Division of Infectious Diseases, University of Colorado Denver, Aurora, CO, United States of America.
  4. Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA, United States of America.
  5. Department of Medicine, University of Zimbabwe College of Health Sciences, Harare, Zimbabwe.
  6. Departments of Medicine and Medical and Laboratory Sciences, University of Zimbabwe College of Health Sciences, Harare, Zimbabwe.

PMID: 27669509 PMCID: PMC5036886 DOI: 10.1371/journal.pone.0163616

Abstract

Diagnosis of KSHV-infected individuals remains a challenge. KSHV prevalence is high in several populations with high prevalence of HIV, leading to increased risk of development of Kaposi's sarcoma (KS). While current assays are reliable for detecting antibodies to KSHV, none are routinely utilized to identify individuals with KSHV infection and thus at increased risk for KS due to assay complexity, lack of access to testing, and cost, particularly in resource-limited settings. Here we describe the addition of KSHV proteins LANA and K8.1 to a previously evaluated HIV/co-infection multiplexed fluorescence immunoassay system. This study demonstrates assay performance by measuring antibody reactivity for KSHV and HIV-1 in a collection of clinical specimens from patients with biopsy-proven KS and sourced negative controls. The KSHV assay correctly identified 155 of 164 plasma samples from patients with biopsy-proven KS and 85 of 93 KSHV antibody (Ab)-negative samples for a sensitivity of 95.1% and specificity of 91.4%. Assay performance for HIV-1 detection was also assessed with 100% agreement with independently verified HIV-1 Ab-positive and Ab-negative samples. These results demonstrate good sensitivity and specificity for detection of antibody to KSHV antigens, and demonstrate the potential for multiplexed co-infection testing in resource-limited settings to identify those at increased risk for HIV-1-related complications.

Conflict of interest statement

This manuscript is a result of the collaboration between authors from the University of California, San Diego, the University of Colorado, and the commercial organization MBio Diagnostics, Inc. Fundin

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