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Sheng F, Chen M, Tan Y, et al. Protective Effects of Otophylloside N on Pentylenetetrazol-Induced Neuronal Injury In vitro and In vivo. Front Pharmacol. 2016;7:224doi: 10.3389/fphar.2016.00224.
Sheng, F., Chen, M., Tan, Y., Xiang, C., Zhang, M., Li, B., Su, H., He, C., Wan, J., & Li, P. (2016). Protective Effects of Otophylloside N on Pentylenetetrazol-Induced Neuronal Injury In vitro and In vivo. Frontiers in pharmacology, 7224. https://doi.org/10.3389/fphar.2016.00224
Sheng, Feiya, et al. "Protective Effects of Otophylloside N on Pentylenetetrazol-Induced Neuronal Injury In vitro and In vivo." Frontiers in pharmacology vol. 7 (2016): 224. doi: https://doi.org/10.3389/fphar.2016.00224
Sheng F, Chen M, Tan Y, Xiang C, Zhang M, Li B, Su H, He C, Wan J, Li P. Protective Effects of Otophylloside N on Pentylenetetrazol-Induced Neuronal Injury In vitro and In vivo. Front Pharmacol. 2016 Jul 25;7:224. doi: 10.3389/fphar.2016.00224. eCollection 2016. PMID: 27504096; PMCID: PMC4959150.
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Front Pharmacol. 2016 Jul 25;7:224. doi: 10.3389/fphar.2016.00224. eCollection 2016.

Protective Effects of Otophylloside N on Pentylenetetrazol-Induced Neuronal Injury In vitro and In vivo.

Frontiers in pharmacology

Feiya Sheng, Mengting Chen, Yuan Tan, Cheng Xiang, Mi Zhang, Baocai Li, Huanxing Su, Chengwei He, Jianbo Wan, Peng Li

Affiliations

  1. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau Macau, China.
  2. Faculty of Life Science and Technology, Kunming University of Science and Technology Kunming, China.

PMID: 27504096 PMCID: PMC4959150 DOI: 10.3389/fphar.2016.00224

Abstract

Approximately 30% of epileptic patients worldwide are medically unable to control their seizures. In addition, repeated epileptic seizures generally lead to neural damage. Pentylenetetrazol (PTZ) is a clinical circulatory and respiratory stimulant that is experimentally used to mimic epileptic convulsion in epilepsy research. Here, we systematically explore the neuroprotective effects of a pure compound isolated from Cynanchum otophyllum Schneid (Qingyangshen), Otophylloside N (OtoN), against PTZ-induced neuronal injury. We used three models: in vitro primary cortical neurons, in vivo mice, and in vivo zebrafish. Our results revealed that OtoN treatment may attenuate PTZ-induced morphology changes, cell death, LDH efflux in embryonic neuronal cells of C57BL/6J mice, and convulsive behavior in zebrafish. Additionally, our Western blot and RT-PCR results demonstrated that OtoN may attenuate PTZ-induced apoptosis and neuronal activation in neuronal cells, mice, and zebrafish. OtoN may reduce PTZ-induced cleavage of poly ADP-ribose polymerase and upregulation of the Bax/Bcl-2 ratio and decrease the expression level of c-Fos. This study is the first investigation of the neuroprotective effects of OtoN, which might be developed as a novel antiepileptic drug.

Keywords: Cynanchum otophyllum Schneid; apoptosis; epilepsy; neuroprotective effect; otophylloside N; pentylenetetrazol

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