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Yi Y, Zhang Y, Ding Y, et al. Simultaneous quantitation of 14 active components in Yinchenhao decoction by using ultra high performance liquid chromatography with diode array detection: Method development and ingredient analysis of different commonly prepared samples. J Sep Sci. 2016;39(21):4147-4157doi: 10.1002/jssc.201600284.
Yi, Y., Zhang, Y., Ding, Y., Lu, L., Zhang, T., Zhao, Y., Xu, X., & Zhang, Y. (2016). Simultaneous quantitation of 14 active components in Yinchenhao decoction by using ultra high performance liquid chromatography with diode array detection: Method development and ingredient analysis of different commonly prepared samples. Journal of separation science, 39(21), 4147-4157. https://doi.org/10.1002/jssc.201600284
Yi, YaXiong, et al. "Simultaneous quantitation of 14 active components in Yinchenhao decoction by using ultra high performance liquid chromatography with diode array detection: Method development and ingredient analysis of different commonly prepared samples." Journal of separation science vol. 39,21 (2016): 4147-4157. doi: https://doi.org/10.1002/jssc.201600284
Yi Y, Zhang Y, Ding Y, Lu L, Zhang T, Zhao Y, Xu X, Zhang Y. Simultaneous quantitation of 14 active components in Yinchenhao decoction by using ultra high performance liquid chromatography with diode array detection: Method development and ingredient analysis of different commonly prepared samples. J Sep Sci. 2016 Nov;39(21):4147-4157. doi: 10.1002/jssc.201600284. Epub 2016 Oct 10. PMID: 27673697.
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J Sep Sci. 2016 Nov;39(21):4147-4157. doi: 10.1002/jssc.201600284. Epub 2016 Oct 10.

Simultaneous quantitation of 14 active components in Yinchenhao decoction by using ultra high performance liquid chromatography with diode array detection: Method development and ingredient analysis of different commonly prepared samples.

Journal of separation science

YaXiong Yi, Yong Zhang, Yue Ding, Lu Lu, Tong Zhang, Yuan Zhao, XiaoJun Xu, YuXin Zhang

Affiliations

  1. School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
  2. Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
  3. Shanghai Environmental Monitoring Center, Shanghai, China.

PMID: 27673697 DOI: 10.1002/jssc.201600284

Abstract

We developed a novel quantitative analysis method based on ultra high performance liquid chromatography coupled with diode array detection for the simultaneous determination of the 14 main active components in Yinchenhao decoction. All components were separated on an Agilent SB-C18 column by using a gradient solvent system of acetonitrile/0.1% phosphoric acid solution at a flow rate of 0.4 mL/min for 35 min. Subsequently, linearity, precision, repeatability, and accuracy tests were implemented to validate the method. Furthermore, the method has been applied for compositional difference analysis of 14 components in eight normal-extraction Yinchenhao decoction samples, accompanied by hierarchical clustering analysis and similarity analysis. The result that all samples were divided into three groups based on different contents of components demonstrated that extraction methods of decocting, refluxing, ultrasonication and extraction solvents of water or ethanol affected component differentiation, and should be related to its clinical applications. The results also indicated that the sample prepared by patients in the family by using water extraction employing a casserole was almost same to that prepared using a stainless-steel kettle, which is mostly used in pharmaceutical factories. This research would help patients to select the best and most convenient method for preparing Yinchenhao decoction.

© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords: Active components; Quantitative analysis; Traditional Chinese medicine; Yinchenhao decoction

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