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Zhao S, Liu W, Li Y, et al. Alternative Splice Variants Modulates Dominant-Negative Function of Helios in T-Cell Leukemia. PLoS One. 2016;11(9):e0163328doi: 10.1371/journal.pone.0163328.
Zhao, S., Liu, W., Li, Y., Liu, P., Li, S., Dou, D., Wang, Y., Yang, R., Xiang, R., & Liu, F. (2016). Alternative Splice Variants Modulates Dominant-Negative Function of Helios in T-Cell Leukemia. PloS one, 11(9), e0163328. https://doi.org/10.1371/journal.pone.0163328
Zhao, Shaorong, et al. "Alternative Splice Variants Modulates Dominant-Negative Function of Helios in T-Cell Leukemia." PloS one vol. 11,9 (2016): e0163328. doi: https://doi.org/10.1371/journal.pone.0163328
Zhao S, Liu W, Li Y, Liu P, Li S, Dou D, Wang Y, Yang R, Xiang R, Liu F. Alternative Splice Variants Modulates Dominant-Negative Function of Helios in T-Cell Leukemia. PLoS One. 2016 Sep 28;11(9):e0163328. doi: 10.1371/journal.pone.0163328. eCollection 2016. PMID: 27681508; PMCID: PMC5040427.
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PLoS One. 2016 Sep 28;11(9):e0163328. doi: 10.1371/journal.pone.0163328. eCollection 2016.

Alternative Splice Variants Modulates Dominant-Negative Function of Helios in T-Cell Leukemia.

PloS one

Shaorong Zhao, Wei Liu, Yinghui Li, Pengjiang Liu, Shufang Li, Daolei Dou, Yue Wang, Rongcun Yang, Rong Xiang, Feifei Liu

Affiliations

  1. Department of Immunology, School of Medicine, Nankai University, Tianjin 300071, China.
  2. Tianjin Entry-Exit Inspection and Quarantine Bureau, Tianjin 300308, China.
  3. Department of Hematology, First-Central Hospital, Tianjin 300060, China.
  4. State Key Laboratory of Medical Chemical Biology, Tianjin 300070, China.
  5. Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Tianjin 300071, China.

PMID: 27681508 PMCID: PMC5040427 DOI: 10.1371/journal.pone.0163328

Abstract

The molecular defects which lead to multistep incidences of human T-cell leukemia have yet to be identified. The DNA-binding protein Helios (known as IKZF2), a member of the Ikaros family of Krüppel-like zinc-finger proteins, functions pivotally in T-cell differentiation and activation. In this study, we identify three novel short Helios splice variants which are T-cell leukemic specific, and demonstrate their dominant-negative function. We then test the cellular localization of distinct Helios isoforms, as well as their capability to form heterodimer with Ikaros, and the association with complexes comprising histone deacetylase (HDAC). In addition, the ectopic expression of T-cell leukemic Helios isoforms interferes with T-cell proliferation and apoptosis. The gene expression profiling and pathway analysis indicated the enrichment of signaling pathways essential for gene expression, translation, cell cycle checkpoint, and response to DNA damage stimulus. These data indicate the molecular function of Helios to be involved in the leukemogenesis and phenotype of T-cell leukemia, and also reveal Helios deregulation as a novel marker for T-cell leukemia.

Conflict of interest statement

The authors have declared that no competing interests exist.

References

  1. Leukemia. 2010 Jul;24(7):1290-8 - PubMed
  2. Leukemia. 2015 Jun;29(6):1301-11 - PubMed
  3. Leuk Res. 2011 Apr;35(4):448-58 - PubMed
  4. Nucleic Acids Res. 2009 Jan;37(Database issue):D674-9 - PubMed
  5. Leukemia. 2010 Jul;24(7):1258-64 - PubMed
  6. Br J Haematol. 2003 Apr;121(1):165-9 - PubMed
  7. Nature. 2015 Oct 22;526(7574):525-30 - PubMed
  8. J Exp Med. 2013 Feb 11;210(2):269-85 - PubMed
  9. Immunity. 1999 Mar;10(3):345-55 - PubMed
  10. Genes Dev. 1998 Mar 15;12(6):782-96 - PubMed
  11. Blood. 2015 Jan 15;125(3):504-15 - PubMed
  12. PLoS One. 2011;6(6):e20731 - PubMed
  13. Nat Immunol. 2014 Mar;15(3):294-304 - PubMed
  14. Science. 2015 Oct 16;350(6258):334-9 - PubMed
  15. Immunol Rev. 2015 Jan;263(1):257-78 - PubMed
  16. Cancer Sci. 2013 Aug;104(8):1097-106 - PubMed
  17. N Engl J Med. 2009 Jan 29;360(5):524-6 - PubMed
  18. Mol Immunol. 2010 Apr;47(7-8):1595-600 - PubMed
  19. Blood. 2007 Mar 1;109(5):2190-7 - PubMed
  20. Immunol Cell Biol. 2003 Jun;81(3):171-5 - PubMed
  21. Nature. 2008 May 1;453(7191):110-4 - PubMed
  22. Biochem Biophys Res Commun. 2011 Mar 18;406(3):430-4 - PubMed
  23. J Cell Biol. 2010 Sep 6;190(5):741-9 - PubMed
  24. Mol Cell Biol. 2005 Oct;25(19):8415-29 - PubMed
  25. J Neurosci Res. 2009 Dec;87(16):3521-34 - PubMed
  26. Cell. 1995 Oct 20;83(2):289-99 - PubMed
  27. J Immunol. 2012 Feb 1;188(3):976-80 - PubMed
  28. Nat Immunol. 2013 Oct;14(10):1073-83 - PubMed
  29. Curr Opin Immunol. 2010 Apr;22(2):154-60 - PubMed
  30. Proc Natl Acad Sci U S A. 2012 Oct 30;109 (44):18072-7 - PubMed
  31. Nat Immunol. 2006 Apr;7(4):382-91 - PubMed
  32. Cancer Sci. 2007 Feb;98(2):182-8 - PubMed
  33. J Immunol. 2006 Mar 15;176(6):3593-602 - PubMed
  34. J Immunol. 2001 Aug 15;167(4):1867-70 - PubMed
  35. Oncogene. 2016 May 12;35(19):2413-27 - PubMed
  36. J Cell Sci. 2007 Aug 1;120(Pt 15):2619-30 - PubMed
  37. Blood. 2001 Nov 1;98(9):2673-80 - PubMed
  38. Curr Biol. 1998 Apr 23;8(9):508-15 - PubMed
  39. EMBO J. 1999 Jun 1;18(11):3090-100 - PubMed
  40. Blood. 2002 Feb 15;99(4):1350-5 - PubMed
  41. Nature. 2011 May 12;473(7346):230-3 - PubMed
  42. Int J Hematol. 2014 Sep;100(3):220-9 - PubMed
  43. Eur J Immunol. 2014 Jul;44(7):2048-58 - PubMed
  44. Mol Cell Biol. 1994 Dec;14(12):8292-303 - PubMed
  45. Nat Immunol. 2011 Jan;12(1):86-95 - PubMed
  46. Leukemia. 2011 Feb;25(2):254-8 - PubMed
  47. J Immunol. 2013 Feb 1;190(3):1008-16 - PubMed
  48. Mol Cell Biol. 2000 Oct;20(20):7572-82 - PubMed
  49. Cell. 1994 Oct 7;79(1):143-56 - PubMed
  50. Immunity. 2010 Nov 24;33(5):685-98 - PubMed

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