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Mousavi Shaegh SA, De Ferrari F, Zhang YS, et al. A microfluidic optical platform for real-time monitoring of pH and oxygen in microfluidic bioreactors and organ-on-chip devices. Biomicrofluidics. 2016;10(4):044111doi: 10.1063/1.4955155.
Mousavi Shaegh, S. A., De Ferrari, F., Zhang, Y. S., Nabavinia, M., Binth Mohammad, N., Ryan, J., Pourmand, A., Laukaitis, E., Banan Sadeghian, R., Nadhman, A., Shin, S. R., Nezhad, A. S., Khademhosseini, A., & Dokmeci, M. R. (2016). A microfluidic optical platform for real-time monitoring of pH and oxygen in microfluidic bioreactors and organ-on-chip devices. Biomicrofluidics, 10(4), 044111. https://doi.org/10.1063/1.4955155
Mousavi Shaegh, Seyed Ali, et al. "A microfluidic optical platform for real-time monitoring of pH and oxygen in microfluidic bioreactors and organ-on-chip devices." Biomicrofluidics vol. 10,4 (2016): 044111. doi: https://doi.org/10.1063/1.4955155
Mousavi Shaegh SA, De Ferrari F, Zhang YS, Nabavinia M, Binth Mohammad N, Ryan J, Pourmand A, Laukaitis E, Banan Sadeghian R, Nadhman A, Shin SR, Nezhad AS, Khademhosseini A, Dokmeci MR. A microfluidic optical platform for real-time monitoring of pH and oxygen in microfluidic bioreactors and organ-on-chip devices. Biomicrofluidics. 2016 Aug 26;10(4):044111. doi: 10.1063/1.4955155. eCollection 2016 Jul. PMID: 27648113; PMCID: PMC5001973.
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Biomicrofluidics. 2016 Aug 26;10(4):044111. doi: 10.1063/1.4955155. eCollection 2016 Jul.

A microfluidic optical platform for real-time monitoring of pH and oxygen in microfluidic bioreactors and organ-on-chip devices.

Biomicrofluidics

Seyed Ali Mousavi Shaegh, Fabio De Ferrari, Yu Shrike Zhang, Mahboubeh Nabavinia, Niema Binth Mohammad, John Ryan, Adel Pourmand, Eleanor Laukaitis, Ramin Banan Sadeghian, Akhtar Nadhman, Su Ryon Shin, Amir Sanati Nezhad, Ali Khademhosseini, Mehmet Remzi Dokmeci

PMID: 27648113 PMCID: PMC5001973 DOI: 10.1063/1.4955155

Abstract

There is a growing interest to develop microfluidic bioreactors and organ-on-chip platforms with integrated sensors to monitor their physicochemical properties and to maintain a well-controlled microenvironment for cultured organoids. Conventional sensing devices cannot be easily integrated with microfluidic organ-on-chip systems with low-volume bioreactors for continual monitoring. This paper reports on the development of a multi-analyte optical sensing module for dynamic measurements of pH and dissolved oxygen levels in the culture medium. The sensing system was constructed using low-cost electro-optics including light-emitting diodes and silicon photodiodes. The sensing module includes an optically transparent window for measuring light intensity, and the module could be connected directly to a perfusion bioreactor without any specific modifications to the microfluidic device design. A compact, user-friendly, and low-cost electronic interface was developed to control the optical transducer and signal acquisition from photodiodes. The platform enabled convenient integration of the optical sensing module with a microfluidic bioreactor. Human dermal fibroblasts were cultivated in the bioreactor, and the values of pH and dissolved oxygen levels in the flowing culture medium were measured continuously for up to 3 days. Our integrated microfluidic system provides a new analytical platform with ease of fabrication and operation, which can be adapted for applications in various microfluidic cell culture and organ-on-chip devices.

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