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Pannkuk EL, Laiakis EC, Mak TD, et al. A Lipidomic and Metabolomic Serum Signature from Nonhuman Primates Exposed to Ionizing Radiation. Metabolomics. 2016;12(5)doi: 10.1007/s11306-016-1010-0.
Pannkuk, E. L., Laiakis, E. C., Mak, T. D., Astarita, G., Authier, S., Wong, K., & Fornace, A. J. (2016). A Lipidomic and Metabolomic Serum Signature from Nonhuman Primates Exposed to Ionizing Radiation. Metabolomics : Official journal of the Metabolomic Society, 12(5), . https://doi.org/10.1007/s11306-016-1010-0
Pannkuk, Evan L, et al. "A Lipidomic and Metabolomic Serum Signature from Nonhuman Primates Exposed to Ionizing Radiation." Metabolomics : Official journal of the Metabolomic Society vol. 12,5 (2016). doi: https://doi.org/10.1007/s11306-016-1010-0
Pannkuk EL, Laiakis EC, Mak TD, Astarita G, Authier S, Wong K, Fornace AJ. A Lipidomic and Metabolomic Serum Signature from Nonhuman Primates Exposed to Ionizing Radiation. Metabolomics. 2016 May;12(5). doi: 10.1007/s11306-016-1010-0. Epub 2016 Mar 15. PMID: 28220056; PMCID: PMC5314995.
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Metabolomics. 2016 May;12(5). doi: 10.1007/s11306-016-1010-0. Epub 2016 Mar 15.

A Lipidomic and Metabolomic Serum Signature from Nonhuman Primates Exposed to Ionizing Radiation.

Metabolomics : Official journal of the Metabolomic Society

Evan L Pannkuk, Evagelia C Laiakis, Tytus D Mak, Giuseppe Astarita, Simon Authier, Karen Wong, Albert J Fornace

Affiliations

  1. Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC.
  2. Mass Spectrometry Data Center, National Institute of Standards and Technology, Gaithersburg, MD.
  3. Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC; Health Sciences, Waters Corporation, Milford, MA.
  4. CiToxLAB North America, Laval, Canada.
  5. Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC; Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC; Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Saudi Arabia.

PMID: 28220056 PMCID: PMC5314995 DOI: 10.1007/s11306-016-1010-0

Abstract

INTRODUCTION: Due to dangers associated with potential accidents from nuclear energy and terrorist threats, there is a need for high-throughput biodosimetry to rapidly assess individual doses of radiation exposure. Lipidomics and metabolomics are becoming common tools for determining global signatures after disease or other physical insult and provide a "snapshot" of potential cellular damage.

OBJECTIVES: The current study assesses changes in the nonhuman primate (NHP) serum lipidome and metabolome 7 days following exposure to ionizing radiation (IR).

METHODS: Serum sample lipids and metabolites were extracted using a biphasic liquid-liquid extraction and analyzed by ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry. Global radiation signatures were acquired in data-independent mode.

RESULTS: Radiation exposure caused significant perturbations in lipid metabolism, affecting all major lipid species, including free fatty acids, glycerolipids, glycerophospholipids and esterified sterols. In particular, we observed a significant increase in the levels of polyunsaturated fatty acids (PUFA)-containing lipids in the serum of NHPs exposed to 10 Gy radiation, suggesting a primary role played by PUFAs in the physiological response to IR. Metabolomics profiling indicated an increase in the levels of amino acids, carnitine, and purine metabolites in the serum of NHPs exposed to 10 Gy radiation, suggesting perturbations to protein digestion/absorption, biological oxidations, and fatty acid β-oxidation.

CONCLUSIONS: This is the first report to determine changes in the global NHP serum lipidome and metabolome following radiation exposure and provides information for developing metabolomic biomarker panels in human-based biodosimetry.

Keywords: Ionizing Radiation; Lipidomics; Metabolomics; Nonhuman Primate

Conflict of interest statement

Conflict of Interest Evan L. Pannkuk, Evagelia C. Laiakis, Tytus D. Mak, Giuseppe Astarita, Simon Authier, Karen Wong, and Albert J. Fornace Jr. declare that they have no conflict of interest.

References

  1. Radiat Res. 2012 May;177(5):717-21 - PubMed
  2. Radiat Res. 2012 Oct;178(4):328-40 - PubMed
  3. Nat Immunol. 2005 Dec;6(12):1191-7 - PubMed
  4. Nucleic Acids Res. 2000 Jan 1;28(1):27-30 - PubMed
  5. Health Phys. 2010 Jun;98(6):863-7 - PubMed
  6. Biochim Biophys Acta. 2007 Jul;1772(7):718-36 - PubMed
  7. Nucleic Acids Res. 2014 Jan;42(Database issue):D472-7 - PubMed
  8. Radiat Res. 1998 Jul;150(1):43-51 - PubMed
  9. J Nutrigenet Nutrigenomics. 2013;6(4-5):181-200 - PubMed
  10. J Proteome Res. 2015 Jan 2;14(1):374-84 - PubMed
  11. Pharmacol Res. 2006 Sep;54(3):165-71 - PubMed
  12. Radiat Prot Dosimetry. 2013 Apr;154(1):9-17 - PubMed
  13. Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6530-5 - PubMed
  14. J Proteome Res. 2014 Sep 5;13(9):4143-54 - PubMed
  15. Chem Rev. 2011 Oct 12;111(10):5944-72 - PubMed
  16. Biochim Biophys Acta. 2012 Sep;1822(9):1442-52 - PubMed
  17. Radiat Environ Biophys. 2014 Nov;53(4):645-57 - PubMed
  18. Radiat Res. 2012 Feb;177(2):187-99 - PubMed
  19. Radiat Res. 2009 Aug;172(2):198-212 - PubMed
  20. Int J Radiat Oncol Biol Phys. 2010 Mar 1;76(3 Suppl):S108-15 - PubMed
  21. J Lipid Res. 2009 Apr;50 Suppl:S9-14 - PubMed
  22. Health Phys. 2012 Oct;103(4):411-26 - PubMed
  23. Radiat Res. 2011 Apr;175(4):473-84 - PubMed
  24. Anal Chem. 2014 Jan 7;86(1):506-13 - PubMed
  25. Alcohol Clin Exp Res. 2014 Jun;38(6):1520-31 - PubMed
  26. J Proteome Res. 2010 Aug 6;9(8):4176-88 - PubMed
  27. Nucleic Acids Res. 2008 Jan;36(Database issue):D344-50 - PubMed
  28. Radiat Res. 2015 Aug;184(2):121-33 - PubMed
  29. Nat Med. 2014 Apr;20(4):415-8 - PubMed
  30. Radiat Res. 2014 Apr;181(4):350-61 - PubMed
  31. Health Phys. 2012 Oct;103(4):427-53 - PubMed
  32. Nucleic Acids Res. 2014 Jan;42(Database issue):D459-71 - PubMed
  33. Int J Radiat Oncol Biol Phys. 2015 Feb 1;91(2):360-7 - PubMed
  34. Nucleic Acids Res. 2007 Jul;35(Web Server issue):W606-12 - PubMed
  35. Nucleic Acids Res. 2009 Jan;37(Database issue):D603-10 - PubMed
  36. Anal Bioanal Chem. 2014 Jul;406(19):4663-75 - PubMed
  37. Am J Clin Nutr. 2006 Jun;83(6 Suppl):1505S-1519S - PubMed
  38. Eur J Nutr. 2013 Aug;52(5):1421-42 - PubMed
  39. Radiat Res. 2014 Jan;181(1):54-64 - PubMed
  40. Nat Rev Mol Cell Biol. 2012 Mar 22;13(4):263-9 - PubMed
  41. PLoS One. 2015 Jun 05;10(6):e0124795 - PubMed
  42. Anal Bioanal Chem. 2012 Sep;404(4):1239-45 - PubMed
  43. Drug Discov Today. 2014 Feb;19(2):164-70 - PubMed
  44. Curr Bioinform. 2012 Mar;7(1):96-108 - PubMed
  45. Biochim Biophys Acta. 2014 Dec;1840(12):3460-3474 - PubMed
  46. J Pathol. 2014 Feb;232(3):289-99 - PubMed
  47. Biochim Biophys Acta. 2015 Apr;1851(4):456-68 - PubMed
  48. Radiat Res. 2008 Jul;170(1):1-14 - PubMed
  49. Anal Chem. 2015 Mar 17;87(6):3177-86 - PubMed
  50. Int J Radiat Biol. 2011 Jan;87(1):91-7 - PubMed
  51. Free Radic Biol Med. 2000 Jun 15;28(12):1751-61 - PubMed
  52. J Biol Chem. 2011 Aug 12;286(32):27855-62 - PubMed

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