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Ji HP, Xiong Y, Zhang ED, et al. Which has more stem-cell characteristics: Müller cells or Müller cells derived from in vivo culture in neurospheres?. Am J Transl Res. 2017;9(2):611-619
Ji, H. P., Xiong, Y., Zhang, E. D., Song, W. T., Gao, Z. L., Yao, F., Sun, H., Zhou, R. R., & Xia, X. B. (2017). Which has more stem-cell characteristics: Müller cells or Müller cells derived from in vivo culture in neurospheres?. American journal of translational research, 9(2), 611-619.
Ji, Hong-Pei, et al. "Which has more stem-cell characteristics: Müller cells or Müller cells derived from in vivo culture in neurospheres?." American journal of translational research vol. 9,2 (2017): 611-619.
Ji HP, Xiong Y, Zhang ED, Song WT, Gao ZL, Yao F, Sun H, Zhou RR, Xia XB. Which has more stem-cell characteristics: Müller cells or Müller cells derived from in vivo culture in neurospheres?. Am J Transl Res. 2017 Feb 15;9(2):611-619. eCollection 2017. PMID: 28337288; PMCID: PMC5340695.
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Am J Transl Res. 2017 Feb 15;9(2):611-619. eCollection 2017.

Which has more stem-cell characteristics: Müller cells or Müller cells derived from in vivo culture in neurospheres?.

American journal of translational research

Hong-Pei Ji, Yu Xiong, En-Dong Zhang, Wei-Tao Song, Zhao-Lin Gao, Fei Yao, Hong Sun, Rong-Rong Zhou, Xiao-Bo Xia

Affiliations

  1. Department of Ophthalmology, Xiangya Hospital, Central South UniversityChangsha 410008, China; Department of Ophthalmology, The People's Hospital of Guizhou ProvinceGuiyang 550002, China.
  2. Department of Ophthalmology, Xiangya Hospital, Central South University Changsha 410008, China.
  3. Department of Oncology, Xiangya Hospital, Central South University Changsha 410008, China.

PMID: 28337288 PMCID: PMC5340695

Abstract

OBJECTIVE: Müller cells can be acquired from in vitro culture or a neurosphere culture system. Both culture methods yield cells with progenitor-cell characteristics that can differentiate into mature nervous cells. We compared the progenitor-cell traits of Müller cells acquired from each method.

METHODS: Primary murine Müller cells were isolated in serum culture media and used to generate Müller cells derived from neurospheres in serum-free culture conditions. Gene expression of neural progenitor cell markers was examined by Q-PCR in the two groups. Expression of rhodopsin and the cone-rod homeobox protein CRX were assessed after induction with 1 μM all-trans retinoic acid (RA) for 7 days.

RESULTS: After more than four passages, many cells were large, flattened, and difficult to passage. A spontaneously immortalized Müller cell line was not established. Three-passage neurospheres yielded few new spheres. Genes coding for Nestin, Sox2, Chx10, and Vimentin were downregulated in cells derived from neurospheres compared to the cells from standard culture, while Pax6 was upregulated. Müller cells from both culture methods were induced into rod photoreceptors, but expression of rhodopsin and CRX was greater in the Müller cells from the standard culture.

CONCLUSION: Both culture methods yielded cells with stem-cell characteristics that can be induced into rod photoreceptor neurons by RA. Serum had no influence on the "stemness" of the cells. Cells from standard culture had greater "stemness" than cells derived from neurospheres. The standard Müller cells would seem to be the best choice for transplantation in cell replacement therapy for photoreceptor degeneration.

Keywords: Müller cells; Stemness; neurospheres

References

  1. Exp Biol Med (Maywood). 2015 May;240(5):682-90 - PubMed
  2. Neuroscience. 2013 Dec 19;254:347-60 - PubMed
  3. Nat Methods. 2006 Oct;3(10):801-6 - PubMed
  4. J Cell Sci. 2009 Sep 1;122(Pt 17):3169-79 - PubMed
  5. Exp Eye Res. 1994 Jun;58(6):675-87 - PubMed
  6. Cytotechnology. 2016 Mar;68(2):267-77 - PubMed
  7. Stem Cell Res Ther. 2013 Aug 14;4(4):94 - PubMed
  8. Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13654-9 - PubMed
  9. Biochem Biophys Res Commun. 2008 Sep 19;374(2):187-91 - PubMed
  10. Mol Vis. 2009;15:713-21 - PubMed
  11. Stem Cells Transl Med. 2014 Mar;3(3):323-33 - PubMed
  12. Biochem Biophys Res Commun. 2010 Apr 16;394(4):877-83 - PubMed
  13. Dongwuxue Yanjiu. 2011 Dec;32(6):611-6 - PubMed
  14. Vision Res. 2008 Jan;48(2):223-34 - PubMed
  15. Invest Ophthalmol Vis Sci. 2009 Jan;50(1):68-74 - PubMed
  16. Nat Neurosci. 2001 Mar;4(3):247-52 - PubMed
  17. Stem Cells. 2007 Aug;25(8):2033-43 - PubMed
  18. Anat Rec (Hoboken). 2013 Sep;296(9):1435-52 - PubMed
  19. Neuroscience. 2012 Dec 6;225:152-61 - PubMed
  20. BMC Dev Biol. 2006 Jul 26;6:36 - PubMed
  21. Proc Natl Acad Sci U S A. 2008 Dec 9;105(49):19508-13 - PubMed
  22. J Biol Chem. 2014 Apr 25;289(17):11945-51 - PubMed
  23. J Neurosci. 2002 Jan 15;22(2):437-45 - PubMed
  24. Stem Cells Dev. 2016 Jan 1;25(1):1-12 - PubMed
  25. Nat Methods. 2005 May;2(5):333-6 - PubMed
  26. Invest Ophthalmol Vis Sci. 2011 Jan 05;52(1):136-45 - PubMed
  27. Nat Rev Neurosci. 2010 Mar;11(3):176-87 - PubMed
  28. Cell Mol Neurobiol. 2015 May;35(4):533-42 - PubMed
  29. BMB Rep. 2015 Apr;48(4):193-9 - PubMed
  30. Neurosci Lett. 2007 Jun 21;421(1):22-6 - PubMed
  31. J Neurosci. 2007 Feb 14;27(7):1712-24 - PubMed
  32. Glia. 2012 Oct;60(10):1579-89 - PubMed

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