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Van Pham P, Vu NB, Dao TT, et al. Extracellular vesicles of ETV2 transfected fibroblasts stimulate endothelial cells and improve neovascularization in a murine model of hindlimb ischemia. Cytotechnology. 2017;69(5):801-814doi: 10.1007/s10616-017-0095-2.
Van Pham, P., Vu, N. B., Dao, T. T., Le, H. T., Phi, L. T., Huynh, O. T., Truong, M. T., Nguyen, O. T., & Phan, N. K. (2017). Extracellular vesicles of ETV2 transfected fibroblasts stimulate endothelial cells and improve neovascularization in a murine model of hindlimb ischemia. Cytotechnology, 69(5), 801-814. https://doi.org/10.1007/s10616-017-0095-2
Van Pham, Phuc, et al. "Extracellular vesicles of ETV2 transfected fibroblasts stimulate endothelial cells and improve neovascularization in a murine model of hindlimb ischemia." Cytotechnology vol. 69,5 (2017): 801-814. doi: https://doi.org/10.1007/s10616-017-0095-2
Van Pham P, Vu NB, Dao TT, Le HT, Phi LT, Huynh OT, Truong MT, Nguyen OT, Phan NK. Extracellular vesicles of ETV2 transfected fibroblasts stimulate endothelial cells and improve neovascularization in a murine model of hindlimb ischemia. Cytotechnology. 2017 Oct;69(5):801-814. doi: 10.1007/s10616-017-0095-2. Epub 2017 May 02. PMID: 28466428; PMCID: PMC5595751.
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Cytotechnology. 2017 Oct;69(5):801-814. doi: 10.1007/s10616-017-0095-2. Epub 2017 May 02.

Extracellular vesicles of ETV2 transfected fibroblasts stimulate endothelial cells and improve neovascularization in a murine model of hindlimb ischemia.

Cytotechnology

Phuc Van Pham, Ngoc Bich Vu, Thuy Thi-Thanh Dao, Ha Thi-Ngan Le, Lan Thi Phi, Oanh Thuy Huynh, Mai Thi-Hoang Truong, Oanh Thi-Kieu Nguyen, Ngoc Kim Phan

Affiliations

  1. Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University, Ho Chi Minh City, Vietnam. [email protected].
  2. Laboratory of Stem Cell Research and Application, University of Science, Vietnam National University, Ho Chi Minh City, Vietnam.

PMID: 28466428 PMCID: PMC5595751 DOI: 10.1007/s10616-017-0095-2

Abstract

Ischemia are common conditions related to lack of blood supply to tissues. Depending on the ischemic sites, ischemia can cause different diseases, such as hindlimb ischemia, heart infarction and stroke. This study aims to evaluate how extracellular vesicles (EVs) derived from ETV2 transfected fibroblasts affect endothelial cell proliferation and neovascularization in a murine model of hindlimb ischemia. Human fibroblasts were isolated and cultured under standard conditions and expanded to the 3th passage before use in experiments. Human fibroblasts were transduced with a viral vector containing the ETV2 gene. Transduced cells were selected by puromycin treatment. These cells were further cultured for collection of EVs, which were isolated from culture supernatant. Following co-culture with endothelial cells, EVs were evaluated for their effect on endothelial cell proliferation and were directly injected into ischemic tissues of a murine model of hindlimb ischemia. The results showed that EVs could induce endothelial cell proliferation in vitro and improved neovascularization in a murine model of hindlimb ischemia. Our results suggest that EVs derived from ETV2-transfected fibroblasts can be promising non-cellular products for the regeneration of blood vessels.

Keywords: ETV2; EVs; Extracellular vesicles; Fibroblasts; Hindlimb ischemia; Ischemia

References

  1. J Cell Biol. 2006 Mar 13;172(6):923-35 - PubMed
  2. PLoS One. 2013 Dec 31;8(12):e84256 - PubMed
  3. Stem Cell Res Ther. 2016 Feb 06;7:24 - PubMed
  4. Dev Biol. 2014 Sep 1;393(1):71-83 - PubMed
  5. Int J Biol Sci. 2016 May 25;12(7):836-49 - PubMed
  6. Cell Biol Int. 2017 Aug;41(8):933 - PubMed
  7. Blood. 2013 May 9;121(19):3997-4006, S1-15 - PubMed
  8. Nat Protoc. 2015 Dec;10(12):1975-85 - PubMed
  9. Cytotherapy. 2015 Apr;17(4):369-81 - PubMed
  10. Cytotherapy. 2016 Feb;18(2):253-62 - PubMed
  11. Stem Cells Dev. 2014 Feb 15;23(4):363-71 - PubMed
  12. Plast Reconstr Surg. 2016 May;137(5):1486-97 - PubMed
  13. FEBS Lett. 2015 May 8;589(11):1257-65 - PubMed
  14. Immunol Lett. 2006 Nov 15;107(2):102-8 - PubMed
  15. Stem Cell Res Ther. 2015 Aug 22;6:148 - PubMed
  16. Eur J Vasc Endovasc Surg. 2016 Jan;51(1):83-9 - PubMed
  17. J Vasc Surg. 2011 Dec;54(6):1650-8 - PubMed
  18. Stem Cells Int. 2015;2015:761643 - PubMed
  19. Oncotarget. 2016 Sep 13;7(37):60687-60697 - PubMed
  20. J Vis Exp. 2016 Jan 31;(107):e53691 - PubMed
  21. J Clin Invest. 2016 Apr 1;126(4):1224-32 - PubMed
  22. Cell Transplant. 2009;18(3):371-80 - PubMed
  23. J Transl Med. 2015 Feb 01;13:49 - PubMed
  24. Circulation. 2015 Mar 10;131(10):851-60 - PubMed
  25. Stem Cells Transl Med. 2016 Dec;5(12 ):1620-1630 - PubMed
  26. Pharmacol Rev. 2012 Jul;64(3):676-705 - PubMed
  27. Biomaterials. 2014 Nov;35(34):9280-9 - PubMed
  28. Stem Cell Res Ther. 2015 Apr 10;6:10 - PubMed
  29. Asian Pac J Cancer Prev. 2013;14(2):987-91 - PubMed
  30. Cytotherapy. 2016 Mar;18(3):413-22 - PubMed
  31. Stem Cells Transl Med. 2016 Oct;5(10 ):1425-1439 - PubMed
  32. Stem Cells Dev. 2015 Jul 15;24(14):1635-47 - PubMed
  33. Arterioscler Thromb Vasc Biol. 2016 Jan;36(1):86-96 - PubMed
  34. Circ Res. 2015 Jun 19;117(1):52-64 - PubMed
  35. Vasa. 2008 Nov;37(4):319-25 - PubMed
  36. Stem Cells Dev. 2013 May 1;22(9):1408-18 - PubMed
  37. Klin Khir. 2014 Jul;(7):42-4 - PubMed
  38. Cell Physiol Biochem. 2014;34(6):1998-2006 - PubMed
  39. Am J Physiol Heart Circ Physiol. 2014 Sep 15;307(6):H869-79 - PubMed
  40. J Dermatol Sci. 2002 Feb;28(2):152-8 - PubMed
  41. Proc Natl Acad Sci U S A. 2015 Jan 6;112(1):160-5 - PubMed
  42. J Mol Cell Cardiol. 2014 Sep;74:139-50 - PubMed
  43. J Control Release. 2016 Sep 28;238:166-175 - PubMed
  44. Stem Cells Int. 2016;2016:7653489 - PubMed
  45. Dev Biol. 2014 May 15;389(2):208-18 - PubMed
  46. J Transl Med. 2013 Jun 10;11:143 - PubMed
  47. J Cell Mol Med. 2016 Jan;20(1):29-37 - PubMed
  48. J Diabetes Complications. 2016 Aug;30(6):986-92 - PubMed
  49. Cancer Lett. 2013 Jul 10;335(1):201-4 - PubMed
  50. Carcinogenesis. 2015 Sep;36(9):1008-18 - PubMed
  51. Am J Pathol. 2015 Aug;185(8):2309-23 - PubMed
  52. Tokai J Exp Clin Med. 2006 Sep 20;31(3):128-32 - PubMed
  53. PLoS One. 2015 May 21;10 (5):e0126715 - PubMed
  54. Mol Ther. 2014 Nov;22(11):1960-70 - PubMed
  55. Stem Cell Res Ther. 2016 Aug 04;7(1):104 - PubMed
  56. Oncoimmunology. 2015 Aug 12;5(4):e1071008 - PubMed
  57. Mol Ther. 2008 Apr;16(4):782-90 - PubMed
  58. Cytotechnology. 2009 Jan;59(1):31-44 - PubMed
  59. Biochem Biophys Res Commun. 2015 Nov 13;467(2):303-9 - PubMed
  60. J Transl Med. 2014 Oct 02;12:274 - PubMed
  61. J Cell Sci. 2016 Jun 1;129(11):2182-9 - PubMed
  62. Cell. 2012 Oct 26;151(3):559-75 - PubMed

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