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Balaiya S, Zhou Z, Chalam KV. Characterization of Vitreous and Aqueous Proteome in Humans With Proliferative Diabetic Retinopathy and Its Clinical Correlation. Proteomics Insights. 2017;8:1178641816686078doi: 10.1177/1178641816686078.
Balaiya, S., Zhou, Z., & Chalam, K. V. (2017). Characterization of Vitreous and Aqueous Proteome in Humans With Proliferative Diabetic Retinopathy and Its Clinical Correlation. Proteomics insights, 81178641816686078. https://doi.org/10.1177/1178641816686078
Balaiya, Sankarathi, et al. "Characterization of Vitreous and Aqueous Proteome in Humans With Proliferative Diabetic Retinopathy and Its Clinical Correlation." Proteomics insights vol. 8 (2017): 1178641816686078. doi: https://doi.org/10.1177/1178641816686078
Balaiya S, Zhou Z, Chalam KV. Characterization of Vitreous and Aqueous Proteome in Humans With Proliferative Diabetic Retinopathy and Its Clinical Correlation. Proteomics Insights. 2017 Mar 15;8:1178641816686078. doi: 10.1177/1178641816686078. eCollection 2017. PMID: 28469465; PMCID: PMC5398322.
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Proteomics Insights. 2017 Mar 15;8:1178641816686078. doi: 10.1177/1178641816686078. eCollection 2017.

Characterization of Vitreous and Aqueous Proteome in Humans With Proliferative Diabetic Retinopathy and Its Clinical Correlation.

Proteomics insights

Sankarathi Balaiya, Zimei Zhou, Kakarla V Chalam

Affiliations

  1. Department of Ophthalmology, College of Medicine, University of Florida, Jacksonville, FL, USA.

PMID: 28469465 PMCID: PMC5398322 DOI: 10.1177/1178641816686078

Abstract

AIMS: Proliferative diabetic retinopathy (PDR) is associated with microvascular complications that cause biochemical changes in the human retina and alter the proteome of vitreous humor and aqueous humor (AH).

METHODS: Human vitreous humor and AH of PDR subjects were collected. Subjects who had surgery for epiretinal membrane or macular hole served as controls. Protein profiles were obtained and analyzed after running the samples on a liquid chromatography-mass spectrometry/mass spectrometry.

RESULTS: In vitreous humor, 16 unique proteins were noted in PDR patients, but not in controls. Those were associated mainly with coagulation, complement, and kallikrein-kinin systems. Under coagulation, fibrinogen and prothrombin proteins were more evident and may emphasize the importance of angiogenesis in the development of PDR. Vitreous proteins showed replicative presence in AH too. As for AH samples, we detected 10 proteins found in PDR patients, which were related to transport, coagulation, and inflammatory responses.

CONCLUSIONS: We found 57 proteins in human vitreous and 39 proteins in AH. Identification of these proteins that are involved in various pathways will be helpful to understand diabetic retinopathy pathogenesis and to develop proteome as a biomarker for PDR.

Keywords: Vitreous and aqueous proteome; humans; proliferative diabetic retinopathy

Conflict of interest statement

DECLARATION OF CONFLICTING INTERESTS: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

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