Display options
Cite
Mao Y, Jiang B, Peng Q, et al. Cloning and characterization of WRKY gene homologs in Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How) and their expression in response to fusaric acid treatment. 3 Biotech. 2017;7(1):86doi: 10.1007/s13205-017-0711-z.
Mao, Y., Jiang, B., Peng, Q., Liu, W., Lin, Y., Xie, D., He, X., & Li, S. (2017). Cloning and characterization of WRKY gene homologs in Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How) and their expression in response to fusaric acid treatment. 3 Biotech, 7(1), 86. https://doi.org/10.1007/s13205-017-0711-z
Mao, Yizhou, et al. "Cloning and characterization of WRKY gene homologs in Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How) and their expression in response to fusaric acid treatment." 3 Biotech vol. 7,1 (2017): 86. doi: https://doi.org/10.1007/s13205-017-0711-z
Mao Y, Jiang B, Peng Q, Liu W, Lin Y, Xie D, He X, Li S. Cloning and characterization of WRKY gene homologs in Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How) and their expression in response to fusaric acid treatment. 3 Biotech. 2017 May;7(1):86. doi: 10.1007/s13205-017-0711-z. Epub 2017 May 13. PMID: 28501986; PMCID: PMC5429887.
Copy Download .nbib Format: NLM
Share it on

3 Biotech. 2017 May;7(1):86. doi: 10.1007/s13205-017-0711-z. Epub 2017 May 13.

Cloning and characterization of WRKY gene homologs in Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How) and their expression in response to fusaric acid treatment.

3 Biotech

Yizhou Mao, Biao Jiang, Qingwu Peng, Wenrui Liu, Yue Lin, Dasen Xie, Xiaoming He, Shaoshan Li

Affiliations

  1. Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
  2. Guangdong Key Lab for New Technology Research of Vegetables, Guangzhou, 510640, China.
  3. Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Science, South China Normal University, Guangzhou, 510631, China.
  4. Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China. [email protected].
  5. Guangdong Key Lab for New Technology Research of Vegetables, Guangzhou, 510640, China. [email protected].
  6. Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Science, South China Normal University, Guangzhou, 510631, China. [email protected].

PMID: 28501986 PMCID: PMC5429887 DOI: 10.1007/s13205-017-0711-z

Abstract

The WRKY transcription factors play an important role in plant resistance for biotic and abiotic stresses. In the present study, we cloned 10 WRKY gene homologs (CqWRKY) in Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua) using the rapid-amplification of cDNA ends (RACE) or homology-based cloning methods. We characterized the structure of these CqWRKY genes. Phylogenetic analysis of these sequences with cucumber homologs suggested possible structural conservation of these genes among cucurbit crops. We examined the expression levels of these genes in response to fusaric acid (FA) treatment between resistant and susceptible Chieh-qua lines with quantitative real-time PCR. All genes could be upregulated upon FA treatment, but four CqWRKY genes exhibited differential expression between resistant and susceptible lines before and after FA application. CqWRKY31 seemed to be a positive regulator while CqWRKY1, CqWRKY23 and CqWRKY53 were negative regulators of fusaric resistance. This is the first report of characterization of WRKY family genes in Chieh-qua. The results may also be useful in breeding Chieh-qua for Fusarium wilt resistance.

Keywords: Benincasa hispida; Chieh-qua; Fusarium resistance; Gene expression; WRKY transcription factor

References

  1. Mol Genet Genomics. 2012 Jun;287(6):495-513 - PubMed
  2. Plant Cell Rep. 2013 Oct;32(10):1589-99 - PubMed
  3. Planta. 2011 Jun;233(6):1237-52 - PubMed
  4. Plant Physiol Biochem. 2012 Nov;60:171-9 - PubMed
  5. Plant Mol Biol. 2003 Jan;51(1):21-37 - PubMed
  6. New Phytol. 2013 Oct;200(2):457-72 - PubMed
  7. Trends Plant Sci. 2000 May;5(5):199-206 - PubMed
  8. BMC Plant Biol. 2008 Jun 20;8:68 - PubMed
  9. Plant Cell Rep. 2015 Aug;34(8):1295-306 - PubMed
  10. Plant Physiol. 2011 Dec;157(4):2081-93 - PubMed
  11. Physiol Plant. 2014 Mar;150(3):397-411 - PubMed
  12. Plant Physiol. 2002 Jun;129(2):706-16 - PubMed
  13. Plant Physiol. 2005 Jan;137(1):176-89 - PubMed
  14. J Plant Physiol. 2007 Aug;164(8):969-79 - PubMed
  15. Bioinformatics. 2007 Nov 1;23(21):2947-8 - PubMed
  16. Indian J Biochem Biophys. 2012 Dec;49(6):405-13 - PubMed
  17. Curr Opin Plant Biol. 2007 Aug;10(4):366-71 - PubMed
  18. BMC Plant Biol. 2012 Aug 12;12:144 - PubMed
  19. Plant Cell Environ. 2013 Apr;36(4):757-74 - PubMed
  20. Plant J. 2006 Nov;48(4):592-605 - PubMed
  21. Mol Plant Pathol. 2013 Feb;14(2):131-44 - PubMed
  22. Cell Res. 2008 Apr;18(4):508-21 - PubMed
  23. BMC Evol Biol. 2005 Jan 03;5:1 - PubMed
  24. BMC Genomics. 2011 Sep 28;12:471 - PubMed
  25. Appl Microbiol Biotechnol. 2008 Jun;79(4):597-603 - PubMed
  26. PLoS One. 2013 Aug 08;8(8):e71054 - PubMed
  27. Mol Plant Pathol. 2014 Sep;15(7):677-89 - PubMed
  28. Indian J Biochem Biophys. 2014 Aug;51(4):302-7 - PubMed
  29. Mol Biol Evol. 2011 Oct;28(10):2731-9 - PubMed
  30. BMC Plant Biol. 2009 Jun 03;9:68 - PubMed
  31. Methods. 2001 Dec;25(4):402-8 - PubMed
  32. DNA Res. 2005 Feb 28;12(1):9-26 - PubMed
  33. Trends Plant Sci. 2010 May;15(5):247-58 - PubMed
  34. Mol Biol Rep. 2011 Jan;38(1):49-58 - PubMed
  35. Plant Mol Biol. 2004 Aug;55(6):853-67 - PubMed

Publication Types