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Hsu CC, Lin CL, Jou IM, et al. The protective role of nitric oxide-dependent innate immunosuppression in the early stage of cartilage damage in rats: Role of nitric oxide in cartilage damage. Bone Joint Res. 2017;6(4):253-258doi: 10.1302/2046-3758.64.BJJ-2016-0161.R1.
Hsu, C. C., Lin, C. L., Jou, I. M., Wang, P. H., & Lee, J. S. (2017). The protective role of nitric oxide-dependent innate immunosuppression in the early stage of cartilage damage in rats: Role of nitric oxide in cartilage damage. Bone & joint research, 6(4), 253-258. https://doi.org/10.1302/2046-3758.64.BJJ-2016-0161.R1
Hsu, C-C, et al. "The protective role of nitric oxide-dependent innate immunosuppression in the early stage of cartilage damage in rats: Role of nitric oxide in cartilage damage." Bone & joint research vol. 6,4 (2017): 253-258. doi: https://doi.org/10.1302/2046-3758.64.BJJ-2016-0161.R1
Hsu CC, Lin CL, Jou IM, Wang PH, Lee JS. The protective role of nitric oxide-dependent innate immunosuppression in the early stage of cartilage damage in rats: Role of nitric oxide in cartilage damage. Bone Joint Res. 2017 Apr;6(4):253-258. doi: 10.1302/2046-3758.64.BJJ-2016-0161.R1. PMID: 28450318; PMCID: PMC5426177.
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Bone Joint Res. 2017 Apr;6(4):253-258. doi: 10.1302/2046-3758.64.BJJ-2016-0161.R1.

The protective role of nitric oxide-dependent innate immunosuppression in the early stage of cartilage damage in rats: Role of nitric oxide in cartilage damage.

Bone & joint research

C-C Hsu, C-L Lin, I-M Jou, P-H Wang, J-S Lee

Affiliations

  1. Department of Orthopedics, College of Medicine, Tainan, Taiwan.
  2. College of Medicine, National Cheng Kung University Hospital, Tainan, Taiwan.
  3. Department of Orthopedics, National Cheng Kung University Hospital, No.138 Sheng-Li Road, 704 Tainan, and Orthopedics Department, E-Da Hospital, No. 1 Yida Road, Jiao-su Village, Yan-Chao District, Kaohsiung City, Taiwan [email protected].
  4. Department of Orthopedics, Chi-Mei Medical Center, No.901, Zhonghua Rd, 710 Tainan, Taiwan.
  5. Department of Neurosurgery, National Cheng Kung University Hospital, No.138 Sheng-Li Road, 704 Tainan, Taiwan.

PMID: 28450318 PMCID: PMC5426177 DOI: 10.1302/2046-3758.64.BJJ-2016-0161.R1

Abstract

OBJECTIVES: Osteoarthritis (OA) is the most common form of arthritis, affecting approximately 15% of the human population. Recently, increased concentration of nitric oxide in serum and synovial fluid in patients with OA has been observed. However, the exact role of nitric oxide in the initiation of OA has not been elucidated. The aim of the present study was to investigate the role of nitric oxide in innate immune regulation during OA initiation in rats.

METHODS: Rat OA was induced by performing meniscectomy surgery while cartilage samples were collected 0, 7, and 14 days after surgery. Cartilage cytokine levels were determined by using enzyme-linked immunosorbent assay, while other proteins were assessed by using Western blot RESULTS: In the time course of the study, nitric oxide was increased seven and 14 days after OA induction. Pro-inflammatory cytokines including tumour necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 were decreased. L-NG-Nitroarginine methyl ester (L-NAME, a non-specific nitric oxide synthase inhibitor) significantly decreased cartilage nitric oxide and blocked immune suppression. Further, L-NAME decreased Matrix metalloproteinase (MMPs) and increased tissue inhibitor of metalloproteinase (TIMP) expression in meniscectomised rats.

CONCLUSION: Nitric oxide-dependent innate immune suppression protects cartilage from damage in the early stages of OA initiation in rats.

© 2017 I-Ming Jou et al.

Keywords: Cartilage; Immunosuppression; Nitric oxide; Osteoarthritis; Rats

Conflict of interest statement

ICMJE Conflicts of Interest: None declared

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