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Lin YS, Lu YL, Wang GJ, et al. Vasorelaxing and antihypertensive activities of synthesized peptides derived from computer-aided simulation of pepsin hydrolysis of yam dioscorin. Bot Stud. 2014;55(1):49doi: 10.1186/s40529-014-0049-3.
Lin, Y. S., Lu, Y. L., Wang, G. J., Liang, H. J., & Hou, W. C. (2014). Vasorelaxing and antihypertensive activities of synthesized peptides derived from computer-aided simulation of pepsin hydrolysis of yam dioscorin. Botanical studies, 55(1), 49. https://doi.org/10.1186/s40529-014-0049-3
Lin, Yin-Shiou, et al. "Vasorelaxing and antihypertensive activities of synthesized peptides derived from computer-aided simulation of pepsin hydrolysis of yam dioscorin." Botanical studies vol. 55,1 (2014): 49. doi: https://doi.org/10.1186/s40529-014-0049-3
Lin YS, Lu YL, Wang GJ, Liang HJ, Hou WC. Vasorelaxing and antihypertensive activities of synthesized peptides derived from computer-aided simulation of pepsin hydrolysis of yam dioscorin. Bot Stud. 2014 Dec;55(1):49. doi: 10.1186/s40529-014-0049-3. Epub 2014 Jun 07. PMID: 28510941; PMCID: PMC5432742.
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Bot Stud. 2014 Dec;55(1):49. doi: 10.1186/s40529-014-0049-3. Epub 2014 Jun 07.

Vasorelaxing and antihypertensive activities of synthesized peptides derived from computer-aided simulation of pepsin hydrolysis of yam dioscorin.

Botanical studies

Yin-Shiou Lin, Yeh-Lin Lu, Guei-Jane Wang, Hong-Jen Liang, Wen-Chi Hou

Affiliations

  1. Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan.
  2. School of Pharmacy, Taipei Medical University, Taipei, Taiwan.
  3. Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan.
  4. Department of Medical Research, China Medical University Hospital, Taichung, Taiwan.
  5. Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
  6. Department of Food Science, Yuanpei University, Hsinchu, Taiwan. [email protected].
  7. Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei, Taiwan. [email protected].
  8. Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei, Taiwan. [email protected].
  9. Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei, Taiwan. [email protected].

PMID: 28510941 PMCID: PMC5432742 DOI: 10.1186/s40529-014-0049-3

Abstract

BACKGROUND: We reported that yam dioscorin and its peptic hydrolysates exhibited ACE inhibition and antihypertensive effects on SHRs, however, the active peptides are not really isolated until now. Using ACE inhibitory screenings, two penta-peptides, KTCGY and KRIHF, were selected for ex vivo and in vivo experiments.

RESULTS: KTCGY, KRIHF, and captopril were shown to have similar vasodilating effects against phenylephrine (PE)-induced tensions in rat endothelium-dependent thoracic aortic rings, however, KTCGYKTCGY (two-repeated KTCGY) and TCGYTCGY (two-repeated TCGY) were showed endothelium-independent vasodilating effects against PE-induced tensions. KTCGY, KRIHF (10 or 20 mg/kg), and captopril (10 mg/kg) were used to evaluate antihypertensive activity during 24-h after a single oral administration to spontaneously hypertensive rats (SHRs). The KTCGY and KRIHF showed significantly different and reduced the systolic blood pressure of SHRs compared to the blank.

CONCLUSIONS: These results suggest that KTCGY and KRIHF may contribute important roles in yam dioscorin for regulating blood pressure in vivo.

Keywords: Angiotensin converting enzyme; Antihypertensive activity; Blood pressure; Vasorelaxing; Yam dioscorin

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