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Woo MY, Yun SJ, Lee MJ, et al. Transcription Factors Regulating Inflammatory Cytokine Production Are Differentially Expressed in Peripheral Blood Mononuclear Cells of Behçet Disease Depending on Disease Activity. Ann Dermatol. 2017;29(2):173-179doi: 10.5021/ad.2017.29.2.173.
Woo, M. Y., Yun, S. J., Lee, M. J., Kim, K., Lee, E. S., & Park, S. (2017). Transcription Factors Regulating Inflammatory Cytokine Production Are Differentially Expressed in Peripheral Blood Mononuclear Cells of Behçet Disease Depending on Disease Activity. Annals of dermatology, 29(2), 173-179. https://doi.org/10.5021/ad.2017.29.2.173
Woo, Min-Yeong, et al. "Transcription Factors Regulating Inflammatory Cytokine Production Are Differentially Expressed in Peripheral Blood Mononuclear Cells of Behçet Disease Depending on Disease Activity." Annals of dermatology vol. 29,2 (2017): 173-179. doi: https://doi.org/10.5021/ad.2017.29.2.173
Woo MY, Yun SJ, Lee MJ, Kim K, Lee ES, Park S. Transcription Factors Regulating Inflammatory Cytokine Production Are Differentially Expressed in Peripheral Blood Mononuclear Cells of Behçet Disease Depending on Disease Activity. Ann Dermatol. 2017 Apr;29(2):173-179. doi: 10.5021/ad.2017.29.2.173. Epub 2017 Mar 24. PMID: 28392644; PMCID: PMC5383742.
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Ann Dermatol. 2017 Apr;29(2):173-179. doi: 10.5021/ad.2017.29.2.173. Epub 2017 Mar 24.

Transcription Factors Regulating Inflammatory Cytokine Production Are Differentially Expressed in Peripheral Blood Mononuclear Cells of Behçet Disease Depending on Disease Activity.

Annals of dermatology

Min-Yeong Woo, Su Jin Yun, Mi Jin Lee, Kyongmin Kim, Eun-So Lee, Sun Park

Affiliations

  1. Department of Microbiology, Ajou University School of Medicine, Suwon, Korea.; Department of Biomedical Sciences, The Graduate School, Ajou University, Suwon, Korea.
  2. Department of Microbiology, Ajou University School of Medicine, Suwon, Korea.
  3. Department of Dermatology, Ajou University School of Medicine, Suwon, Korea.

PMID: 28392644 PMCID: PMC5383742 DOI: 10.5021/ad.2017.29.2.173

Abstract

BACKGROUND: Behçet disease (BD) is a relapsing inflammatory disease with increased production of inflammatory cytokines in peripheral blood mononuclear cells (PBMCs); however, the underlying molecular mechanisms are not well known.

OBJECTIVE: To analyze whether the differential expression of transcription factors is involved in the increased tumor necrosis factor (TNF)-α and interleukin (IL)-6 production by PBMCs of BD patients compared to healthy controls (HCs).

METHODS: Expression of transcription factors was examined by real-time reverse transcriptase-polymerase chain reaction and western blotting. Cytokine production by CD11b+ cells transfected with siRNAs against transcription factors was measured by enzyme-linked immunosorbent assay.

RESULTS: In the absence of lipopolysaccharide stimulation, the transcript level of CCAAT-enhancer-binding proteins (C/EBP) β was increased in PBMCs from patients with active BD compared to that in PBMCs from patients with stable BD. The C/EBPδ transcript level was higher in PBMCs from patients with active BD than in those from HCs. The activating transcription factor 3 (ATF3) transcript level was increased in PBMCs from patients with stable BD compared to that in PBMCs from HCs. siRNAs targeting C/EBPβ and C/EBPδ significantly reduced the production of IL-6 and TNF-α in lipopolysaccharide-stimulated CD11b+ cells from patients with BD as well as from HCs.

CONCLUSION: We found differential expression of C/EBPβ, C/EBPδ, and ATF3 in PBMCs from patients with BD depending on disease activity, indicating the involvement of these molecules in BD pathogenesis.

Keywords: Behcet syndrome; CCAAT-enhancer-binding proteins; Gene expression; Interleukin-6; Tumor necrosis factor-alpha

Conflict of interest statement

CONFLICTS OF INTEREST: The authors have nothing to disclose.

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