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Wang K, Niu Y, Wang Q, et al. Cloning and evaluation of reference genes for quantitative real-time PCR analysis in . PeerJ. 2017;5:e3260doi: 10.7717/peerj.3260.
Wang, K., Niu, Y., Wang, Q., Liu, H., Jin, Y., & Zhang, S. (2017). Cloning and evaluation of reference genes for quantitative real-time PCR analysis in . PeerJ, 5e3260. https://doi.org/10.7717/peerj.3260
Wang, Kai, et al. "Cloning and evaluation of reference genes for quantitative real-time PCR analysis in ." PeerJ vol. 5 (2017): e3260. doi: https://doi.org/10.7717/peerj.3260
Wang K, Niu Y, Wang Q, Liu H, Jin Y, Zhang S. Cloning and evaluation of reference genes for quantitative real-time PCR analysis in . PeerJ. 2017 Apr 26;5:e3260. doi: 10.7717/peerj.3260. eCollection 2017. PMID: 28462052; PMCID: PMC5408727.
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PeerJ. 2017 Apr 26;5:e3260. doi: 10.7717/peerj.3260. eCollection 2017.

Cloning and evaluation of reference genes for quantitative real-time PCR analysis in .

PeerJ

Kai Wang, Yi Niu, Qijun Wang, Haili Liu, Yi Jin, Shenglin Zhang

Affiliations

  1. College of Horticulture and Landscape, Southwest University, Chongqing, China.
  2. Ministry of Education, Key Laboratory of Horticulture Science for Southern Mountainous Regions, Southwest University, Chongqing, China.
  3. Chongqing Education Commission, Konjac Resource Utilization Engineering Research in Chongqing Colleges and Universities, Chongqing, China.

PMID: 28462052 PMCID: PMC5408727 DOI: 10.7717/peerj.3260

Abstract

Quantitative real-time reverse transcription PCR (RT-qPCR) has been widely used in the detection and quantification of gene expression levels because of its high accuracy, sensitivity, and reproducibility as well as its large dynamic range. However, the reliability and accuracy of RT-qPCR depends on accurate transcript normalization using stably expressed reference genes.

Keywords: Amorphophallus; Gene expression; Monocotyledon; Real-time reverse transcription PCR; Reference genes

Conflict of interest statement

The authors declare there are no completing interests.

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