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Zheng J, Choo K, Bradt C, et al. Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder. Biotechnol Rep (Amst). 2014;3:99-107doi: 10.1016/j.btre.2014.06.008.
Zheng, J., Choo, K., Bradt, C., Lehoux, R., & Rehmann, L. (2014). Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder. Biotechnology reports (Amsterdam, Netherlands), 399-107. https://doi.org/10.1016/j.btre.2014.06.008
Zheng, Jun, et al. "Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder." Biotechnology reports (Amsterdam, Netherlands) vol. 3 (2014): 99-107. doi: https://doi.org/10.1016/j.btre.2014.06.008
Zheng J, Choo K, Bradt C, Lehoux R, Rehmann L. Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder. Biotechnol Rep (Amst). 2014 Jun 26;3:99-107. doi: 10.1016/j.btre.2014.06.008. eCollection 2014 Sep. PMID: 28626653; PMCID: PMC5466104.
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Biotechnol Rep (Amst). 2014 Jun 26;3:99-107. doi: 10.1016/j.btre.2014.06.008. eCollection 2014 Sep.

Enzymatic hydrolysis of steam exploded corncob residues after pretreatment in a twin-screw extruder.

Biotechnology reports (Amsterdam, Netherlands)

Jun Zheng, Kim Choo, Chris Bradt, Rick Lehoux, Lars Rehmann

Affiliations

  1. Department of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond Street, London, ON N6A 3K7, Canada.
  2. Lambton College, 1457 London Road, Sarnia, ON N7S 6K4, Canada.
  3. GreenField Specialty Alcohols Inc. 275 Bloomfield Road, Chatham, ON N7 M 0N6, Canada.

PMID: 28626653 PMCID: PMC5466104 DOI: 10.1016/j.btre.2014.06.008

Abstract

A modified twin-screw extruder incorporated with a filtration device was used as a liquid/solid separator for xylose removal from steam exploded corncobs. A face centered central composite design was used to study the combined effects of various enzymatic hydrolysis process variables (enzyme loading, surfactant addition, and hydrolysis time) with two differently extruded corncobs (7% xylose removal, 80% xylose removal) on glucose conversion. The results showed that the extrusion process led to an increase in cellulose crystallinity, while structural changes could also be observed via SEM. A quadratic polynomial model was developed for predicting the glucose conversion and the fitted model provided an adequate approximation of the true response as verified by the analysis of variance (ANOVA).

Keywords: Bioethanol; Lignocellulosic biomass; Mechanical pretreatment

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