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Domínguez-Martín E, Cardenal-Muñoz E, King JS, et al. Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum. Cells. 2017;6(3)doi: 10.3390/cells6030018.
Domínguez-Martín, E., Cardenal-Muñoz, E., King, J. S., Soldati, T., Coria, R., & Escalante, R. (2017). Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum. Cells, 6(3), . https://doi.org/10.3390/cells6030018
Domínguez-Martín, Eunice, et al. "Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum." Cells vol. 6,3 (2017). doi: https://doi.org/10.3390/cells6030018
Domínguez-Martín E, Cardenal-Muñoz E, King JS, Soldati T, Coria R, Escalante R. Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum. Cells. 2017 Jul 03;6(3). doi: 10.3390/cells6030018. PMID: 28671610; PMCID: PMC5617964.
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Cells. 2017 Jul 03;6(3). doi: 10.3390/cells6030018.

Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum.

Cells

Eunice Domínguez-Martín, Elena Cardenal-Muñoz, Jason S King, Thierry Soldati, Roberto Coria, Ricardo Escalante

Affiliations

  1. Instituto de Investigaciones Biomédicas "Alberto Sols" (CSIC-UAM), Arturo Duperier 4, 28029 Madrid, Spain. [email protected].
  2. Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico. [email protected].
  3. Département de Biochimie, Faculté des Sciences, Université de Genève, Sciences II, CH-1211-Genève-4, Switzerland. [email protected].
  4. Department of Biomedical Sciences and Bateson Centre, University of Sheffield, Sheffield S10 2TN, UK. [email protected].
  5. Département de Biochimie, Faculté des Sciences, Université de Genève, Sciences II, CH-1211-Genève-4, Switzerland. [email protected].
  6. Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico. [email protected].
  7. Instituto de Investigaciones Biomédicas "Alberto Sols" (CSIC-UAM), Arturo Duperier 4, 28029 Madrid, Spain. [email protected].

PMID: 28671610 PMCID: PMC5617964 DOI: 10.3390/cells6030018

Abstract

Autophagy is a eukaryotic catabolic pathway that degrades and recycles cellular components to maintain homeostasis. It can target protein aggregates, superfluous biomolecular complexes, dysfunctional and damaged organelles, as well as pathogenic intracellular microbes. Autophagy is a dynamic process in which the different stages from initiation to final degradation of cargo are finely regulated. Therefore, the study of this process requires the use of a palette of techniques, which are continuously evolving and whose interpretation is not trivial. Here, we present the social amoeba

Keywords: Dictyostelium; autophagic markers; autophagy; cleavage assays; flux assays

Conflict of interest statement

The authors declare no conflict of interest.

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