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Ersozlu S, Sarisozen B, Ozer O, et al. The biochemical and histological analysis of subcutaneous calcitonin and intramedullary methylprednisolone on bone repair after bone marrow ablation: an experimental comparative study in rats. J Exp Orthop. 2017;4(1):25doi: 10.1186/s40634-017-0100-x.
Ersozlu, S., Sarisozen, B., Ozer, O., Adim, S. B., & Sahin, O. (2017). The biochemical and histological analysis of subcutaneous calcitonin and intramedullary methylprednisolone on bone repair after bone marrow ablation: an experimental comparative study in rats. Journal of experimental orthopaedics, 4(1), 25. https://doi.org/10.1186/s40634-017-0100-x
Ersozlu, Salim, et al. "The biochemical and histological analysis of subcutaneous calcitonin and intramedullary methylprednisolone on bone repair after bone marrow ablation: an experimental comparative study in rats." Journal of experimental orthopaedics vol. 4,1 (2017): 25. doi: https://doi.org/10.1186/s40634-017-0100-x
Ersozlu S, Sarisozen B, Ozer O, Adim SB, Sahin O. The biochemical and histological analysis of subcutaneous calcitonin and intramedullary methylprednisolone on bone repair after bone marrow ablation: an experimental comparative study in rats. J Exp Orthop. 2017 Dec;4(1):25. doi: 10.1186/s40634-017-0100-x. Epub 2017 Jul 20. PMID: 28730582; PMCID: PMC5519512.
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J Exp Orthop. 2017 Dec;4(1):25. doi: 10.1186/s40634-017-0100-x. Epub 2017 Jul 20.

The biochemical and histological analysis of subcutaneous calcitonin and intramedullary methylprednisolone on bone repair after bone marrow ablation: an experimental comparative study in rats.

Journal of experimental orthopaedics

Salim Ersozlu, Bartu Sarisozen, Ozgur Ozer, Saduman Balaban Adim, Orcun Sahin

Affiliations

  1. Faculty of Medicine, Department of Orthopaedics and Traumatology, Istinye University, Liv Hospital, ?stanbul, Turkey. [email protected].
  2. Faculty of Medicine, Department of Orthopaedics and Traumatology, Uludag University, Bursa, Turkey.
  3. Faculty of Medicine, Department of Pathology, Uludag University, Bursa, Turkey.
  4. Faculty of Medicine, Department of Orthopaedics and Traumatology, Baskent University, Ankara, Turkey.

PMID: 28730582 PMCID: PMC5519512 DOI: 10.1186/s40634-017-0100-x

Abstract

BACKGROUND: Although, glucocorticoid (GC) and calcitonin-induced changes in bone repair have been studied previously, the exact effects of these on fracture healing remain controversial. Hence, the purpose of this experimental study is to determine biochemical and histological effects of locally administrated GC and systemically administrated calcitonin on the kinetics of healing response after bone marrow ablation in rats.

METHODS: After having undergone marrow ablation, a steroid-treated group of rats (n = 24) received a single dose of intramedullary methylprednisolone (2 mg/kg), a calcitonin-treated group (n = 24) received intermittently administrated subcutaneous salmon calcitonin (16 IU/kg), and a control group (n = 24) received intramedullary saline (25 μl).

RESULTS: Blood samples taken on days 1, 3, 7, 9, and 15 after ablation showed an increase in serum calcium, alkaline phosphatase (ALP), and phosphate levels in the Calcitonin and Control groups. Levels of calcium and ALP peaked on day 7 after ablation. However, an increase in phosphate levels indicated a biphasic reaction that peaked on the third and ninth day after ablation. Hypercalcemia was not observed in Steroid group because of the inhibition of osteoclastic bone resorption. In that group, the serum levels of ALP and phosphate were lower than baseline levels. The levels of urinary calcium excretion peaked 3 to 7 days after marrow ablation in the control group and 7 to 9 days after that procedure in the steroid group. Histologic evaluation showed that the rats in the control group demonstrated the expected healing period according to the histological grades and that a delay in healing occurred in the calcitonin group after day 9 because of the inhibition of osteoclastic bone resorption. All rats in the steroid group exhibited a decrease and delayed healing response.

CONCLUSION: Total serum calcium, phosphate, and ALP levels increased after bilateral tibial bone marrow ablation and urine calcium and hydroxyproline excretion also increased as a factor of bone resorption. Subcutaneously administrated salmon calcitonin did not affect biochemical changes after marrow ablation. Single-dose intramedullary methylprednisolone inhibited extra-tibial bone resorption induced by cytokines after bone marrow ablation.

Keywords: Bone repair; Calcitonin; Fracture healing; Marrow ablation; Methylprednisolone

References

  1. J Bone Miner Res. 1997 Feb;12(2):200-9 - PubMed
  2. Bone. 2000 Jul;27(1):103-10 - PubMed
  3. Bone. 1996 Apr;18(4):375-80 - PubMed
  4. Mol Med Rep. 2015 Aug;12 (2):1717-26 - PubMed
  5. Connect Tissue Res. 1989;23(2-3):153-61 - PubMed
  6. Calcif Tissue Int. 2005 Oct;77(4):212-25 - PubMed
  7. J Bone Miner Res. 1994 Jan;9(1):69-73 - PubMed
  8. Bone. 1990;11(1):29-33 - PubMed
  9. Horm Res. 1987;27(4):200-4 - PubMed
  10. Bone Miner. 1989 Jan;5(2):193-200 - PubMed
  11. Calcif Tissue Int. 1992 Apr;50(4):381-3 - PubMed
  12. Bone. 1989;10 (1):53-60 - PubMed
  13. Clin Orthop Relat Res. 1974 May;(100):349-55 - PubMed
  14. Am J Pathol. 2012 Jul;181(1):234-44 - PubMed
  15. Osteoporos Int. 2016 Apr;27(4):1493-505 - PubMed
  16. Calcif Tissue Int. 1984 Jul;36(4):357-60 - PubMed
  17. Int J Rad Appl Instrum B. 1988;15(4):469-76 - PubMed
  18. Clin Orthop Relat Res. 1985 Oct;(199):272-9 - PubMed
  19. Clin Orthop Relat Res. 1981 Jun;(157):225-57 - PubMed
  20. Bone. 2015 Oct;79:222-32 - PubMed
  21. Jpn J Pharmacol. 2002 Nov;90(3):236-46 - PubMed
  22. Mol Med Rep. 2016 Aug;14 (2):1054-60 - PubMed
  23. Clin Orthop Relat Res. 1982 Sep;(169):219-42 - PubMed
  24. Clin Orthop Relat Res. 1984 Jul-Aug;(187):289-95 - PubMed
  25. Osteoporos Int. 2015 Jan;26(1):383-93 - PubMed
  26. J Clin Endocrinol Metab. 1981 Jan;52(1):111-5 - PubMed
  27. Bone. 1992;13(6):435-41 - PubMed
  28. Metabolism. 1983 Feb;32(2):151-6 - PubMed
  29. J Bone Joint Surg Br. 1993 Jul;75(4):645-9 - PubMed
  30. J Bone Miner Res. 1993 Mar;8(3):379-88 - PubMed
  31. Endocrinology. 1990 May;126(5):2607-13 - PubMed
  32. Calcif Tissue Int. 1995 Apr;56(4):274-9 - PubMed

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