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Tang Q, Liu Y, Tsytsarev V, et al. High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT). Biomed Opt Express. 2017;8(4):2124-2137doi: 10.1364/BOE.8.002124.
Tang, Q., Liu, Y., Tsytsarev, V., Lin, J., Wang, B., Kanniyappan, U., Li, Z., & Chen, Y. (2017). High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT). Biomedical optics express, 8(4), 2124-2137. https://doi.org/10.1364/BOE.8.002124
Tang, Qinggong, et al. "High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT)." Biomedical optics express vol. 8,4 (2017): 2124-2137. doi: https://doi.org/10.1364/BOE.8.002124
Tang Q, Liu Y, Tsytsarev V, Lin J, Wang B, Kanniyappan U, Li Z, Chen Y. High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT). Biomed Opt Express. 2017 Mar 09;8(4):2124-2137. doi: 10.1364/BOE.8.002124. eCollection 2017 Apr 01. PMID: 28736659; PMCID: PMC5516817.
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Biomed Opt Express. 2017 Mar 09;8(4):2124-2137. doi: 10.1364/BOE.8.002124. eCollection 2017 Apr 01.

High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT).

Biomedical optics express

Qinggong Tang, Yi Liu, Vassiliy Tsytsarev, Jonathan Lin, Bohan Wang, Udayakumar Kanniyappan, Zhifang Li, Yu Chen

Affiliations

  1. Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA.
  2. Contributed equally.
  3. Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, MD 21201 USA.
  4. Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, College of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou 350007, China.

PMID: 28736659 PMCID: PMC5516817 DOI: 10.1364/BOE.8.002124

Abstract

Three-dimensional fluorescence laminar optical tomography (FLOT) can achieve resolutions of 100-200 µm and penetration depths of 2-3 mm. FLOT has been used in tissue engineering, neuroscience, as well as oncology. The limited dynamic range of the charge-coupled device-based system makes it difficult to image fluorescent samples with a large concentration difference, limits its penetration depth, and diminishes the quantitative accuracy of 3D reconstruction data. Here, incorporating the high-dynamic-range (HDR) method widely used in digital cameras, we present HDR-FLOT, increasing penetration depth and improving the ability to image fluorescent samples with a large concentration difference. The method was tested using an agar phantom and a B6 mouse for brain imaging

Keywords: (170.2520) Fluorescence microscopy; (170.3010) Image reconstruction techniques; (170.3880) Medical and biological imaging; (170.6960) Tomography

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