Display options
Cite
Zheng J, Yi D, Liu Y, et al. Long nonding RNA UCA1 regulates neural stem cell differentiation by controlling miR-1/Hes1 expression. Am J Transl Res. 2017;9(8):3696-3704
Zheng, J., Yi, D., Liu, Y., Wang, M., Zhu, Y., & Shi, H. (2017). Long nonding RNA UCA1 regulates neural stem cell differentiation by controlling miR-1/Hes1 expression. American journal of translational research, 9(8), 3696-3704.
Zheng, Jiaolin, et al. "Long nonding RNA UCA1 regulates neural stem cell differentiation by controlling miR-1/Hes1 expression." American journal of translational research vol. 9,8 (2017): 3696-3704.
Zheng J, Yi D, Liu Y, Wang M, Zhu Y, Shi H. Long nonding RNA UCA1 regulates neural stem cell differentiation by controlling miR-1/Hes1 expression. Am J Transl Res. 2017 Aug 15;9(8):3696-3704. eCollection 2017. PMID: 28861160; PMCID: PMC5575183.
Copy Download .nbib Format: NLM
Share it on

Am J Transl Res. 2017 Aug 15;9(8):3696-3704. eCollection 2017.

Long nonding RNA UCA1 regulates neural stem cell differentiation by controlling miR-1/Hes1 expression.

American journal of translational research

Jiaolin Zheng, Dan Yi, Yu Liu, Mingqiu Wang, Yulan Zhu, Huaizhang Shi

Affiliations

  1. Department of Neruology, The Second Hospital of Harbin Medical UniversityHarbin 150086, Heilong Jiang, China.
  2. Department of Pharmacology, Rush University Medical CenterChicago IL 60612, USA.
  3. Department of Neurosurgery, The First Hospital of Harbin Medical UniversityHarbin 150001, Heilong Jiang, China.

PMID: 28861160 PMCID: PMC5575183

Abstract

Neural stem cells are able to self-renew and generate glial and neuronal lineages. Neural stem cell may serve as therapeutic method for neurological disorders including spinal cord injuries, Parkinson's disease, Huntington's disease and Alzheimer's disease. Long noncoding RNAs (lncRNAs) are longer than 200 nucleotides with limited protein-coding capacity. Recent studies have demonstreated that lncRNAs play an important role in several cellular processes including cell differentiation, cell development, proliferation, apoptosis, invasion and migration. However, the role of lncRNA human urothelial carcinoma associated 1 (UCA1) in the development of neural stem cells remains unknown. In this study, we showed that the expression of UCA1 was upregulated in the neural stem cell in a time-dependent manner. Knockdown of UCA1 suppressed the neural stem cell proliferation. Inhibition of UCA1 decreased the expression of nestin and the formation of neurosphere. Moreover, knockdown of UCA1 suppressed the neural stem cell differentiation to astrocyte and promoted the neural stem cell differentiation to neuron. Furthermore, we demonstrated that knockdown of UCA1 increased the expression of miR-1 in the neural stem cell and suppressed the expresion of Hes1, which is one target gene of miR-1. In addition, ectopic expression of Hes1 could impair siUCA1-induced neural stem cells proliferation. Overexpression of Hes1 suppressed siUCA1-induced β-tubulin expression and promoted siUCA1-inhibited GFAP expression in the neural stem cell. These results suggested that UCA1 regulated the neural stem cell proliferation and differentiation through regulating Hes1 expression.

Keywords: Hes1; LncRNAs; Neural stem cells; UCA1; miR-1

Conflict of interest statement

None.

References

  1. Int J Clin Exp Med. 2015 Aug 15;8(8):12609-16 - PubMed
  2. Biomed Res Int. 2013;2013:216286 - PubMed
  3. Med Oncol. 2016 Aug;33(8):88 - PubMed
  4. J Exp Clin Cancer Res. 2015 Feb 19;34:18 - PubMed
  5. RNA Biol. 2014;11(9):1105-12 - PubMed
  6. PLoS One. 2013 Sep 17;8(9):e73991 - PubMed
  7. Cancer Chemother Pharmacol. 2016 May;77(5):1061-7 - PubMed
  8. Cell Death Dis. 2015 Jan 08;6:e1583 - PubMed
  9. Curr Alzheimer Res. 2016;13(11):1219-1231 - PubMed
  10. Asian Pac J Cancer Prev. 2013;14(2):1077-82 - PubMed
  11. Eur Rev Med Pharmacol Sci. 2016 Jul;20(13):2819-24 - PubMed
  12. Cancer Lett. 2013 Oct 10;339(2):159-66 - PubMed
  13. Biochem Biophys Res Commun. 2015 Nov 13;467(2):223-8 - PubMed
  14. Tumour Biol. 2015 Mar;36(3):1643-51 - PubMed
  15. Tumour Biol. 2014 Oct;35(10):10075-84 - PubMed
  16. Clin Cancer Res. 2006 Aug 15;12(16):4851-8 - PubMed
  17. Mol Med Rep. 2016 Sep;14 (3):2067-76 - PubMed
  18. PLoS One. 2010 Oct 18;5(10):e13453 - PubMed
  19. Diagn Pathol. 2015 Mar 27;10:14 - PubMed
  20. Crit Rev Oncol Hematol. 2008 Jan;65(1):43-53 - PubMed
  21. Tumour Biol. 2016 Aug;37(8):10633-41 - PubMed
  22. Cell Death Dis. 2016 Aug 11;7(8):e2327 - PubMed
  23. Oncotarget. 2015 Aug 14;6(23):19759-79 - PubMed
  24. PLoS One. 2013 Jun 27;8(6):e67411 - PubMed
  25. Arch Iran Med. 2016 Jul;19(7):508-17 - PubMed
  26. Oncol Lett. 2015 Oct;10(4):1953-1958 - PubMed
  27. Nucleic Acids Res. 2013 Apr 1;41(6):3699-712 - PubMed
  28. Eur Rev Med Pharmacol Sci. 2015 Sep;19(18):3403-11 - PubMed
  29. Int J Clin Exp Pathol. 2015 Aug 01;8(8):9440-5 - PubMed
  30. J Toxicol Environ Health A. 2016;79(9-10):407-18 - PubMed
  31. Int J Clin Exp Pathol. 2015 May 01;8(5):5427-34 - PubMed
  32. Mol Cell Neurosci. 2010 Jan;43(1):127-35 - PubMed
  33. Mol Med Rep. 2015 Oct;12(4):5611-8 - PubMed
  34. EMBO J. 2013 Oct 30;32(21):2819-32 - PubMed
  35. Cardiology. 2013;125(1):18-30 - PubMed
  36. BMC Neurosci. 2012 Sep 28;13:116 - PubMed
  37. J Cancer Res Clin Oncol. 2013 Mar;139(3):437-45 - PubMed
  38. Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):1876-81 - PubMed
  39. Sci Rep. 2016 Apr 05;6:23892 - PubMed
  40. Biochem J. 2016 Oct 15;473(20):3639-3654 - PubMed
  41. Anticancer Res. 2015 Oct;35(10):5377-82 - PubMed
  42. FEBS Lett. 2008 Jun 11;582(13):1919-27 - PubMed
  43. PLoS One. 2011;6(8):e21396 - PubMed
  44. Circ Res. 2014 Apr 25;114(9):1389-97 - PubMed
  45. Stem Cells Dev. 2014 Feb 15;23(4):393-405 - PubMed
  46. Development. 2015 Feb 1;142(3):477-85 - PubMed
  47. Biomed Pharmacother. 2014 Jun;68(5):557-64 - PubMed
  48. Cancer Lett. 2016 Feb 1;371(1):99-106 - PubMed
  49. Cell Death Dis. 2014 May 22;5:e1243 - PubMed
  50. Stem Cells Int. 2016;2016:9745315 - PubMed

Publication Types