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Xie JW, Zhao Y, Feng XL, et al. [Protective effect of butylphthalide in a cell model of Alzheimer's disease induced by Aβ25-35 in Neuro 2a cells]. Nan Fang Yi Ke Da Xue Xue Bao. 2017;37(9):1228-1233
Xie, J. W., Zhao, Y., Feng, X. L., Lin, F. B., Luo, J., Liu, X., & Hou, D. R. (2017). [Protective effect of butylphthalide in a cell model of Alzheimer's disease induced by Aβ25-35 in Neuro 2a cells]. Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 37(9), 1228-1233.
Xie, Jing-Wen, et al. "[Protective effect of butylphthalide in a cell model of Alzheimer's disease induced by Aβ25-35 in Neuro 2a cells]." Nan fang yi ke da xue xue bao = Journal of Southern Medical University vol. 37,9 (2017): 1228-1233.
Xie JW, Zhao Y, Feng XL, Lin FB, Luo J, Liu X, Hou DR. [Protective effect of butylphthalide in a cell model of Alzheimer's disease induced by Aβ25-35 in Neuro 2a cells]. Nan Fang Yi Ke Da Xue Xue Bao. 2017 Sep 20;37(9):1228-1233. Chinese. PMID: 28951367; PMCID: PMC6765483.
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Nan Fang Yi Ke Da Xue Xue Bao. 2017 Sep 20;37(9):1228-1233.

[Protective effect of butylphthalide in a cell model of Alzheimer's disease induced by Aβ25-35 in Neuro 2a cells].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University

[Article in Chinese]
Jing-Wen Xie, Yan Zhao, Xia-Lu Feng, Fang-Bo Lin, Jing Luo, Xin Liu, De-Ren Hou

Affiliations

  1. Department of Neurology, Third Xiangya Hospital of Central South University, Changsha 410013, China.E-mail: [email protected].

PMID: 28951367 PMCID: PMC6765483

Abstract

OBJECTIVE: To study the protective effect of butylphthalide in a cell model of Alzheimer's disease(AD) induced by Aβ25-35 in Neuro 2a (N2a) cells.

METHODS: N2a cells were divided into AD group, butylphthalide (NBP) group and control group. AD cell model was established by adding 20 µmol/L Aβ25-35 to cultured N2a cells. The cells in NBP group were treated with 0.1, 1, 10, or 100 µmol/L NBP 4 h prior to treatment with 20 µmol/L Aβ25-35. The cell viability were determined by MTT assay, the cell apoptotic rate were detected by AnnexinV-FITC flow cytometry, and the cell morphological changes were observed under inverted phase contrast microscope. The expression of TNF-α and IL-1β mRNA were determined by qRT-PCR.

RESULTS: Compared with those in the control group, the number of adherent cells was significantly decreased, neurite structures were reduced, and the cell viability was decreased, while the apoptotic rate and expressions of TNF-α and IL-1β mRNA were increased in AD group (P<0.05). Compared with that in AD group, the number of adherent cells was increased in NBP group and the cell morphology was similar to the normal control cells. The cell viability of N2a cells was increased in NBP group with decreased apoptotic rate and expression of TNF-αand IL-1β mRNA (P<0.05).

CONCLUSION: Butylphthalide can protect against AD in the cell model induced by Aβ25-35 possibly by inhibiting the expression of inflammatory cytokines.

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