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Lindgren G, Wennerberg J, Ekblad L. Cell line dependent expression of EpCAM influences the detection of circulating tumor cells with CellSearch. Laryngoscope Investig Otolaryngol. 2017;2(4):194-198doi: 10.1002/lio2.83.
Lindgren, G., Wennerberg, J., & Ekblad, L. (2017). Cell line dependent expression of EpCAM influences the detection of circulating tumor cells with CellSearch. Laryngoscope investigative otolaryngology, 2(4), 194-198. https://doi.org/10.1002/lio2.83
Lindgren, Gustaf, et al. "Cell line dependent expression of EpCAM influences the detection of circulating tumor cells with CellSearch." Laryngoscope investigative otolaryngology vol. 2,4 (2017): 194-198. doi: https://doi.org/10.1002/lio2.83
Lindgren G, Wennerberg J, Ekblad L. Cell line dependent expression of EpCAM influences the detection of circulating tumor cells with CellSearch. Laryngoscope Investig Otolaryngol. 2017 May 20;2(4):194-198. doi: 10.1002/lio2.83. eCollection 2017 Aug. PMID: 28894840; PMCID: PMC5562942.
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Laryngoscope Investig Otolaryngol. 2017 May 20;2(4):194-198. doi: 10.1002/lio2.83. eCollection 2017 Aug.

Cell line dependent expression of EpCAM influences the detection of circulating tumor cells with CellSearch.

Laryngoscope investigative otolaryngology

Gustaf Lindgren, Johan Wennerberg, Lars Ekblad

Affiliations

  1. Division of Oto-rhino-laryngology, Head and Neck Surgery Lund Sweden.
  2. Department of Clinical Sciences Lund Skåne University Hospital, Lund University Lund Sweden.
  3. Division of Oncology and Pathology Lund Sweden.
  4. Department of Clinical Sciences Lund Faculty of Medicine, Lund University Lund Sweden.

PMID: 28894840 PMCID: PMC5562942 DOI: 10.1002/lio2.83

Abstract

OBJECTIVES: The existence of circulating tumor cells has emerged as an important factor for prognosis and survival. The CellSearch method is the only circulating tumor cell detection method approved by the US Food and Drug Administration for clinical use. It relies on the detection of EpCAM (epithelial cell adhesion molecule) and is approved for colon cancer, breast cancer and prostate cancer. We now investigated whether CellSearch can be used to quantify circulating tumor cells in head and neck squamous cell cancer.

STUDY DESIGN AND METHODS: In this study, we investigated the expression of EpCAM in 12 head and neck squamous cell cancer cell lines using Western blot and how this affected their detectability with CellSearch in peripheral blood.

RESULTS: We found a great variation in the expression of EpCAM between our head and neck squamous cell cancer cell lines. This was accompanied by variations in counting efficiency.

CONCLUSION: We suggest that for reliable quantification of circulating tumor cells in blood from patients with head and neck squamous cell cancer cell, an epitope independent method is preferable.

LEVEL OF EVIDENCE: NA.

Keywords: CellSearch; EpCAM; Head and neck squamous cell carcinoma; circulating tumor cells; cytokeratin

References

  1. Clin Cancer Res. 2008 Oct 1;14(19):6302-9 - PubMed
  2. Lancet Oncol. 2014 Apr;15(4):406-14 - PubMed
  3. PLoS One. 2014 Dec 05;9(12):e113706 - PubMed
  4. J Clin Oncol. 2008 Jul 1;26(19):3213-21 - PubMed
  5. Cancer Genet Cytogenet. 2002 Jan 15;132(2):85-96 - PubMed
  6. Sci Rep. 2015 Jul 17;5:12270 - PubMed
  7. J Pathol. 2001 Jul;194(3):298-302 - PubMed
  8. Clin Cancer Res. 2006 Jul 15;12(14 Pt 1):4218-24 - PubMed
  9. Oral Oncol. 2010 Jul;46(7):485-91 - PubMed
  10. J Proteome Res. 2011 Mar 4;10(3):1416-9 - PubMed
  11. Anticancer Res. 2006 Jan-Feb;26(1A):153-8 - PubMed
  12. J Cell Biol. 1997 Dec 1;139(5):1337-48 - PubMed
  13. Clin Cancer Res. 2016 May 15;22(10):2583-93 - PubMed
  14. Int J Radiat Oncol Biol Phys. 2012 Aug 1;83(5):e685-90 - PubMed
  15. Oncotarget. 2016 Nov 1;7(44):71223-71234 - PubMed
  16. N Engl J Med. 2004 Aug 19;351(8):781-91 - PubMed
  17. BMC Cancer. 2016 Jul 08;16:433 - PubMed
  18. Med Oncol. 2015 May;32(5):164 - PubMed
  19. PLoS One. 2015 Jul 17;10(7):e0132901 - PubMed
  20. Clin Cancer Res. 2004 Oct 15;10(20):6897-904 - PubMed
  21. Ann Oncol. 2009 Jul;20(7):1223-9 - PubMed
  22. Anal Chem. 2015 Sep 15;87(18):9322-8 - PubMed
  23. Anticancer Res. 1999 May-Jun;19(3B):2237-42 - PubMed
  24. Int J Cancer. 2015 Jun 1;136(11):2515-23 - PubMed
  25. Ann Transl Med. 2014 Nov;2(11):108 - PubMed
  26. Anticancer Res. 2015 Jul;35(7):3953-60 - PubMed
  27. BMC Cancer. 2013 Jan 25;13:33 - PubMed
  28. Int J Cancer. 2010 Dec 15;127(12):2893-917 - PubMed
  29. Eur Arch Otorhinolaryngol. 2013 Sep;270(10 ):2745-9 - PubMed
  30. Laryngoscope. 2016 Jun;126(6):E213-7 - PubMed
  31. Ann Oncol. 2011 Aug;22(8):1878-85 - PubMed
  32. Head Neck. 2012 Oct;34(10):1440-4 - PubMed
  33. Arch Otolaryngol Head Neck Surg. 2010 Dec;136(12):1274-9 - PubMed

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