Display options
Cite
Suh KS, Chon S, Choi EM. Bergenin increases osteogenic differentiation and prevents methylglyoxal-induced cytotoxicity in MC3T3-E1 osteoblasts. Cytotechnology. 2017;70(1):215-224doi: 10.1007/s10616-017-0135-y.
Suh, K. S., Chon, S., & Choi, E. M. (2018). Bergenin increases osteogenic differentiation and prevents methylglyoxal-induced cytotoxicity in MC3T3-E1 osteoblasts. Cytotechnology, 70(1), 215-224. https://doi.org/10.1007/s10616-017-0135-y
Suh, Kwang Sik, et al. "Bergenin increases osteogenic differentiation and prevents methylglyoxal-induced cytotoxicity in MC3T3-E1 osteoblasts." Cytotechnology vol. 70,1 (2018): 215-224. doi: https://doi.org/10.1007/s10616-017-0135-y
Suh KS, Chon S, Choi EM. Bergenin increases osteogenic differentiation and prevents methylglyoxal-induced cytotoxicity in MC3T3-E1 osteoblasts. Cytotechnology. 2018 Feb;70(1):215-224. doi: 10.1007/s10616-017-0135-y. Epub 2017 Sep 11. PMID: 28895006; PMCID: PMC5809652.
Copy Download .nbib Format: NLM
Share it on

Cytotechnology. 2018 Feb;70(1):215-224. doi: 10.1007/s10616-017-0135-y. Epub 2017 Sep 11.

Bergenin increases osteogenic differentiation and prevents methylglyoxal-induced cytotoxicity in MC3T3-E1 osteoblasts.

Cytotechnology

Kwang Sik Suh, Suk Chon, Eun Mi Choi

Affiliations

  1. Department of Endocrinology and Metabolism, School of Medicine, Kyung Hee University, 1, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
  2. Department of Endocrinology and Metabolism, School of Medicine, Kyung Hee University, 1, Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea. [email protected].

PMID: 28895006 PMCID: PMC5809652 DOI: 10.1007/s10616-017-0135-y

Abstract

Bergenin, an active component of plants in the genus Bergenia, has multiple biological activities, including anti-inflammatory and immunomodulatory properties. We investigated the effects of bergenin on MC3T3-E1 osteoblasts. Bergenin treatment significantly elevated collagen synthesis, alkaline phosphatase activity, osteocalcin synthesis, and mineralization in the cells (p < 0.05). Additionally, bergenin increased the ratio of osteoprotegerin to receptor activator of nuclear factor kappa-B ligand, and cyclophilin B release. Methylglyoxal (MG), a highly reactive dicarbonyl compound, is the major precursor in the formation of advanced glycation end products. Pretreatment of MC3T3-E1 cells with bergenin prevented MG-induced cell death. Furthermore, bergenin treatment significantly reduced the induction of activating transcription factor 6 and autophagy by MG. These results indicate that bergenin may have positive effects on critical osteoblastic cell functions.

Keywords: Bergenin; Cytotoxicity; Differentiation; Methylglyoxal; Osteoblasts

References

  1. Biochem J. 1999 Nov 15;344 Pt 1:109-16 - PubMed
  2. Physiol Res. 2003;52(6):657-79 - PubMed
  3. Eur J Endocrinol. 2016 Apr;174(4):R127-38 - PubMed
  4. Histochem Cell Biol. 1999 Oct;112(4):271-6 - PubMed
  5. Cell Death Differ. 2007 Sep;14(9):1576-82 - PubMed
  6. Planta Med. 2002 Apr;68(4):372-4 - PubMed
  7. J Biol Chem. 1995 Aug 4;270(31):18323-8 - PubMed
  8. Nature. 2003 May 15;423(6937):337-42 - PubMed
  9. J Ethnopharmacol. 2007 Jun 13;112(2):401-5 - PubMed
  10. Biochem Pharmacol. 2012 Dec 15;84(12):1660-70 - PubMed
  11. Autophagy. 2008 Feb;4(2):246-8 - PubMed
  12. Cell. 1997 Apr 18;89(2):309-19 - PubMed
  13. Oxid Med Cell Longev. 2016;2016:1051925 - PubMed
  14. Endocrinology. 2001 Dec;142(12):5050-5 - PubMed
  15. Endocr Rev. 2013 Oct;34(5):658-90 - PubMed
  16. J Asian Nat Prod Res. 2005 Feb;7(1):13-8 - PubMed
  17. PLoS Genet. 2014 Jun 26;10(6):e1004465 - PubMed
  18. J Ethnopharmacol. 2000 Oct;72 (3):469-74 - PubMed
  19. Curr Drug Targets. 2005 Jun;6(4):453-74 - PubMed
  20. J Biol Chem. 2001 Dec 28;276(52):48967-72 - PubMed
  21. Nature. 2006 Oct 19;443(7113):780-6 - PubMed
  22. Int J Exp Pathol. 1999 Dec;80(6):305-15 - PubMed
  23. Dev Cell. 2008 Dec;15(6):829-40 - PubMed
  24. Cell Signal. 2013 Feb;25(2):552-60 - PubMed
  25. Mutat Res. 2005 Jan 6;569(1-2):29-63 - PubMed
  26. Genes Dev. 2002 Jun 1;16(11):1345-55 - PubMed
  27. Can J Diabetes. 2014 Apr;38(2):94-100 - PubMed
  28. Chin J Nat Med. 2014 Dec;12(12):929-36 - PubMed
  29. Curr Top Med Chem. 2003;3(12):1315-47 - PubMed
  30. Cell Death Differ. 2009 Aug;16(8):1093-107 - PubMed
  31. Trends Genet. 2004 Jan;20(1):33-43 - PubMed
  32. Genome Biol. 2005;6(7):226 - PubMed
  33. Biochim Biophys Acta. 2009 Jul;1792(7):651-63 - PubMed
  34. Osteoporos Int. 2011 Jan;22(1):1-20 - PubMed
  35. Planta Med. 2004 May;70(5):421-6 - PubMed
  36. Free Radic Res. 2014 Feb;48(2):206-17 - PubMed
  37. Mol Cell Neurosci. 1999 Sep;14(3):180-98 - PubMed
  38. Histol Histopathol. 2004 Oct;19(4):1325-44 - PubMed
  39. Am J Hum Genet. 2009 Oct;85(4):521-7 - PubMed
  40. Diabetes. 2008 Jan;57(1):41-9 - PubMed
  41. J Nat Prod. 1996 Jun;59(6):565-9 - PubMed
  42. Curr Osteoporos Rep. 2007 Sep;5(3):98-104 - PubMed
  43. Bone. 2007 May;40(5):1408-14 - PubMed
  44. Mol Cell. 2000 Dec;6(6):1355-64 - PubMed
  45. Bone. 2009 May;44(5):936-41 - PubMed
  46. Neuroscience. 2002;115(2):587-602 - PubMed
  47. Yao Xue Xue Bao. 1981 Jun;16(6):425-8 - PubMed
  48. Nature. 1996 Aug 1;382(6590):448-52 - PubMed
  49. Autophagy. 2008 Jul 1;4(5):600-606 - PubMed

Publication Types