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Mukherjee M, Jones JC, Yao J. Lumbosacral stenosis in Labrador retriever military working dogs - an exomic exploratory study. Canine Genet Epidemiol. 2017;4:12doi: 10.1186/s40575-017-0052-6.
Mukherjee, M., Jones, J. C., & Yao, J. (2017). Lumbosacral stenosis in Labrador retriever military working dogs - an exomic exploratory study. Canine genetics and epidemiology, 412. https://doi.org/10.1186/s40575-017-0052-6
Mukherjee, Meenakshi, et al. "Lumbosacral stenosis in Labrador retriever military working dogs - an exomic exploratory study." Canine genetics and epidemiology vol. 4 (2017): 12. doi: https://doi.org/10.1186/s40575-017-0052-6
Mukherjee M, Jones JC, Yao J. Lumbosacral stenosis in Labrador retriever military working dogs - an exomic exploratory study. Canine Genet Epidemiol. 2017 Oct 23;4:12. doi: 10.1186/s40575-017-0052-6. eCollection 2017. PMID: 29085643; PMCID: PMC5651560.
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Canine Genet Epidemiol. 2017 Oct 23;4:12. doi: 10.1186/s40575-017-0052-6. eCollection 2017.

Lumbosacral stenosis in Labrador retriever military working dogs - an exomic exploratory study.

Canine genetics and epidemiology

Meenakshi Mukherjee, Jeryl C Jones, Jianbo Yao

Affiliations

  1. Departments of Animal and Nutritional Sciences, Davis College of Agriculture, Natural Resources and Design, West Virginia University, Morgantown, WV 26506 USA.
  2. Current address: 140 Poole Agricultural Center, Department of Animal and Veterinary Sciences, Clemson University, Clemson, 29634 USA.

PMID: 29085643 PMCID: PMC5651560 DOI: 10.1186/s40575-017-0052-6

Abstract

BACKGROUND: Canine lumbosacral stenosis is defined as narrowing of the caudal lumbar and/or sacral vertebral canal. A risk factor for neurologic problems in many large sized breeds, lumbosacral stenosis can also cause early retirement in Labrador retriever military working dogs. Though vital for conservative management of the condition, early detection is complicated by the ambiguous nature of clinical signs of lumbosacral stenosis in stoic and high-drive Labrador retriever military working dogs. Though clinical diagnoses of lumbosacral stenosis using CT imaging are standard, they are usually not performed unless dogs present with clinical symptoms. Understanding the underlying genomic mechanisms would be beneficial in developing early detection methods for lumbosacral stenosis, which could prevent premature retirement in working dogs. The exomes of 8 young Labrador retriever military working dogs (4 affected and 4 unaffected by lumbosacral stenosis, phenotypically selected by CT image analyses from 40 dogs with no reported clinical signs of the condition) were sequenced to identify and annotate exonic variants between dogs negative and positive for lumbosacral stenosis.

RESULTS: Two-hundred and fifty-two variants were detected to be homozygous for the wild allele and either homozygous or heterozygous for the variant allele. Seventeen non-disruptive variants were detected that could affect protein effectiveness in 7 annotated (SCN1B, RGS9BP, ASXL3, TTR, LRRC16B, PTPRO, ZBBX) and 3 predicted genes (EEF1A1, DNAJA1, ZFX). No exonic variants were detected in any of the canine orthologues for human lumbar spinal stenosis candidate genes.

CONCLUSIONS: TTR (transthyretin) gene could be a possible candidate for lumbosacral stenosis in Labrador retrievers based on previous human studies that have reported an association between human lumbar spinal stenosis and transthyretin protein amyloidosis. Other genes identified with exonic variants in this study but with no known published association with lumbosacral stenosis and/or lumbar spinal stenosis could also be candidate genes for future canine lumbosacral stenosis studies but their roles remain currently unknown. Human lumbar spinal stenosis candidate genes also cannot be ruled out as lumbosacral stenosis candidate genes. More definitive genetic investigations of this condition are needed before any genetic test for lumbosacral stenosis in Labrador retriever can be developed.

Keywords: CT; Canine; Cauda equina syndrome; Computed tomography; Genetics; Genomics; Lumbar spinal stenosis; Military working dogs; Transthyretin; Whole exome sequencing

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