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Schoonen L, van Esterik KS, Zhang C, et al. Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis. Sci Rep. 2017;7(1):14772doi: 10.1038/s41598-017-15310-y.
Schoonen, L., van Esterik, K. S., Zhang, C., Ulijn, R. V., Nolte, R. J. M., & Hest, J. C. M. V. (2017). Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis. Scientific reports, 7(1), 14772. https://doi.org/10.1038/s41598-017-15310-y
Schoonen, Lise, et al. "Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis." Scientific reports vol. 7,1 (2017): 14772. doi: https://doi.org/10.1038/s41598-017-15310-y
Schoonen L, van Esterik KS, Zhang C, Ulijn RV, Nolte RJM, Hest JCMV. Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis. Sci Rep. 2017 Nov 07;7(1):14772. doi: 10.1038/s41598-017-15310-y. PMID: 29116178; PMCID: PMC5676709.
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Sci Rep. 2017 Nov 07;7(1):14772. doi: 10.1038/s41598-017-15310-y.

Alternative application of an affinity purification tag: hexahistidines in ester hydrolysis.

Scientific reports

Lise Schoonen, Kayleigh S van Esterik, Chunqiu Zhang, Rein V Ulijn, Roeland J M Nolte, Jan C M van Hest

Affiliations

  1. Department of Bio-Organic Chemistry, Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands.
  2. Advanced Science Research Center, City University of New York, 85 St Nicholas Terrace, New York, 10031, USA.
  3. Department of Bio-Organic Chemistry, Eindhoven University of Technology, PO Box 513 (STO 3.31), 5600 MB Eindhoven, Eindhoven, The Netherlands. [email protected].
  4. Department of Bio-Organic Chemistry, Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands. [email protected].

PMID: 29116178 PMCID: PMC5676709 DOI: 10.1038/s41598-017-15310-y

Abstract

Hexahistidines are very common tags used in the affinity chromatography purification of recombinant proteins. Although these tags are solely applied for their metal-binding properties, we found that they are also able to perform ester hydrolysis when attached to a protein. For instance, green fluorescent protein (GFP) and the cowpea chlorotic mottle virus (CCMV) are able to perform catalysis after introduction of the His-tag. By attaching a His-tag to an enzyme, a dual-functional catalyst was created, that can perform a two-step cascade reaction. These findings show that the catalytic properties of the hexahistidine tag should be taken into consideration when choosing a suitable protein purification tag.

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