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Plants (Basel). 2017 Oct 24;6(4). doi: 10.3390/plants6040052.

In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes.

Plants (Basel, Switzerland)

Csengele E Barta, Bethany Bolander, Steven R Bilby, Jeremy H Brown, Reid N Brown, Alexander M Duryee, Danielle R Edelman, Christina E Gray, Chandler Gossett, Amie G Haddock, Mackenzie M Helsel, Alyssa D Jones, Marissa E Klingseis, Kalif Leslie, Edward W Miles, Rachael A Prawitz

Affiliations

  1. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  2. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  3. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  4. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  5. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  6. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  7. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  8. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  9. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  10. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  11. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  12. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  13. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  14. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  15. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].
  16. Department of Biology, Missouri Western State University, 4525 Downs Drive, Agenstein-Remington Halls, St. Joseph, MO 64507, USA. [email protected].

PMID: 29073736 PMCID: PMC5750628 DOI: 10.3390/plants6040052

Abstract

Mature oak (

Keywords: 18S rRNA; DNA extraction; PCR; dark adaptation; gene identification; isoprene synthase (IspS); leaves; pin oak (Quercus palustris); secondary metabolites

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