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Wang H, Li Y, Zhang K, et al. Feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of Penicillium novae-zelandiae. AMB Express. 2018;8(1):4doi: 10.1186/s13568-017-0529-4.
Wang, H., Li, Y., Zhang, K., Ma, Y., & Li, P. (2018). Feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of Penicillium novae-zelandiae. AMB Express, 8(1), 4. https://doi.org/10.1186/s13568-017-0529-4
Wang, Hailei, et al. "Feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of Penicillium novae-zelandiae." AMB Express vol. 8,1 (2018): 4. doi: https://doi.org/10.1186/s13568-017-0529-4
Wang H, Li Y, Zhang K, Ma Y, Li P. Feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of Penicillium novae-zelandiae. AMB Express. 2018 Jan 08;8(1):4. doi: 10.1186/s13568-017-0529-4. PMID: 29313259; PMCID: PMC5758489.
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AMB Express. 2018 Jan 08;8(1):4. doi: 10.1186/s13568-017-0529-4.

Feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of Penicillium novae-zelandiae.

AMB Express

Hailei Wang, Yi Li, Kun Zhang, Yingqun Ma, Ping Li

Affiliations

  1. Henan Province Engineering Laboratory for Bioconversion Technology of Functional Microbes, College of Life Sciences, Henan Normal University, Xinxiang, 453007, China.
  2. Advanced Environmental Biotechnology Center, Nanyang Environment and Water Research Institute, Nanyang Technological University, Singapore, 637141, Singapore.
  3. Henan Province Engineering Laboratory for Bioconversion Technology of Functional Microbes, College of Life Sciences, Henan Normal University, Xinxiang, 453007, China. [email protected].

PMID: 29313259 PMCID: PMC5758489 DOI: 10.1186/s13568-017-0529-4

Abstract

In this study, a biomembrane surface fermentation was used to produce red pigments of Penicillium novae-zelandiae, and the significant improvement in pigment production by the addition of 0.4 g/L of tyrosine demonstrated that the red pigments probably contained betalain. Therefore, one red pigment was purified, and identified as 2-decarboxybetanin by high-resolution mass spectrometry (MS) and MS/MS analysis. Transcriptomic analysis revealed the differentially expressed genes and metabolic profile of P. novae-zelandiae in response to different cultivations and exhibited the complete biosynthetic pathway of 2-decarboxybetanin in P. novae-zelandiae. Betalains are important water-soluble nitrogen-containing food coloring agents, obtained mainly from beetroot by chemical extraction. This paper is the first report about the production of betalain by microbial fermentation, and results exhibit the possible use of fungal fermentation in future 2-decarboxybetanin production.

Keywords: Betalains; Biomembrane surface cultivation; Penicillium novae-zelandiae; RNA sequencing; Tyrosine

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