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Wang S, Bruning A, Jeon O, et al. An . Biomicrofluidics. 2018;12(1):014106doi: 10.1063/1.5017644.
Wang, S., Bruning, A., Jeon, O., Long, F., Alsberg, E., & Choi, C. K. (2018). An . Biomicrofluidics, 12(1), 014106. https://doi.org/10.1063/1.5017644
Wang, Shuo, et al. "An ." Biomicrofluidics vol. 12,1 (2018): 014106. doi: https://doi.org/10.1063/1.5017644
Wang S, Bruning A, Jeon O, Long F, Alsberg E, Choi CK. An . Biomicrofluidics. 2018 Jan 10;12(1):014106. doi: 10.1063/1.5017644. eCollection 2018 Jan. PMID: 29375727; PMCID: PMC5762234.
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Biomicrofluidics. 2018 Jan 10;12(1):014106. doi: 10.1063/1.5017644. eCollection 2018 Jan.

An .

Biomicrofluidics

Shuo Wang, Andrew Bruning, Oju Jeon, Fei Long, Eben Alsberg, Chang Kyoung Choi

Affiliations

  1. Department of Mechanical Engineering-Engineering Mechanics, Michigan Technological University, Houghton, Michigan 49931, USA.
  2. Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA.

PMID: 29375727 PMCID: PMC5762234 DOI: 10.1063/1.5017644

Abstract

Alginate microgels are widely generated by ionic crosslinking methods, but this method has limitations in controlling the microgel degradation and generating non-spherical microgels. By employing oxidized methacrylated alginate (OMA) that is degradable and photocrosslinkable, we have successfully photocrosslinked monodisperse OMA microgels and demonstrated the feasibility to generate discoid alginate microgels. However, several technical issues obstructed our opto-microfluidic method from being a useful technique. Here, we further characterized and optimized this method. Monodisperse discoid OMA microgels with good shape consistency were, for the first time, generated. The curability of OMA microgels was characterized as the macromer concentration varied from 2% to 10%, and the minimum required photoinitiator (VA-086) concentrations were determined. The effects of crosslinking density and the presence of ions in the storage solution on swelling of OMA hydrogels were identified to give insights into accurate controlling of the microgel size. A much quicker degradation rate (within three weeks) compared to ionically crosslinked alginate hydrogels was indirectly identified by quantifying the elastic modulus using atomic force microscopy. The viability of encapsulated chondrocytes in OMA microgels formed by this method was higher than those from other existing methods, demonstrating its favorable cytocompatibility. It was found that the oxygen tension played a critical role in both the curability of microgels and the cytocompatibility of this technique. We also summarize common practical issues and provide related solutions and/or operational suggestions. By this method, OMA microgels are expected to be valuable alternatives to traditional ionically crosslinked alginate microgels in drug delivery, tissue engineering, and single cell analysis areas due to their multiple favorable properties.

References

  1. Angew Chem Int Ed Engl. 2005 Jan 21;44(5):724-8 - PubMed
  2. Anal Chem. 1998 Dec 1;70(23):4974-84 - PubMed
  3. Acta Biomater. 2012 May;8(5):1838-48 - PubMed
  4. Lab Chip. 2011 Mar 7;11(5):966-8 - PubMed
  5. Nat Protoc. 2013 May;8(5):870-91 - PubMed
  6. Langmuir. 2006 Oct 24;22(22):9453-7 - PubMed
  7. Ultramicroscopy. 2001 Jan;86(1-2):207-15 - PubMed
  8. Trends Biotechnol. 1998 May;16(5):224-30 - PubMed
  9. J Biosci Bioeng. 2010 Jun;109(6):618-21 - PubMed
  10. Biomicrofluidics. 2012 Nov 07;6(4):44108 - PubMed
  11. Prog Polym Sci. 2012 Jan;37(1):106-126 - PubMed
  12. Biomaterials. 2005 Jun;26(16):3327-31 - PubMed
  13. Tissue Eng Part C Methods. 2011 Feb;17(2):173-9 - PubMed
  14. Biomaterials. 2012 May;33(13):3503-14 - PubMed
  15. Biotechnol Bioeng. 1996 May 20;50(4):374-81 - PubMed
  16. J Biomech. 2007;40(5):1011-23 - PubMed
  17. Macromol Biosci. 2006 Aug 7;6(8):623-33 - PubMed
  18. Biomicrofluidics. 2016 Feb 03;10 (1):011101 - PubMed
  19. Ind Eng Chem Res. 2015 Apr 29;54(16):4043-4059 - PubMed

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