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Huang W, Yao L, He X, et al. Hypoglycemic activity and constituents analysis of blueberry (. Diabetes Metab Syndr Obes. 2018;11:357-366doi: 10.2147/DMSO.S166728.
Huang, W., Yao, L., He, X., Wang, L., Li, M., Yang, Y., & Wan, C. (2018). Hypoglycemic activity and constituents analysis of blueberry (. Diabetes, metabolic syndrome and obesity : targets and therapy, 11357-366. https://doi.org/10.2147/DMSO.S166728
Huang, Weifeng, et al. "Hypoglycemic activity and constituents analysis of blueberry (." Diabetes, metabolic syndrome and obesity : targets and therapy vol. 11 (2018): 357-366. doi: https://doi.org/10.2147/DMSO.S166728
Huang W, Yao L, He X, Wang L, Li M, Yang Y, Wan C. Hypoglycemic activity and constituents analysis of blueberry (. Diabetes Metab Syndr Obes. 2018 Jul 17;11:357-366. doi: 10.2147/DMSO.S166728. eCollection 2018. PMID: 30046248; PMCID: PMC6054273.
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Diabetes Metab Syndr Obes. 2018 Jul 17;11:357-366. doi: 10.2147/DMSO.S166728. eCollection 2018.

Hypoglycemic activity and constituents analysis of blueberry (.

Diabetes, metabolic syndrome and obesity : targets and therapy

Weifeng Huang, Liangliang Yao, Xiao He, Lei Wang, Mingxi Li, Youxin Yang, Chunpeng Wan

Affiliations

  1. Department of Microbiology and Immunology, Medical College, China Three Gorge University, Yichang, Hubei 443002, China.
  2. Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330006, China.
  3. College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China, [email protected].
  4. Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, Nanchang 330045, China, [email protected].
  5. Collaborative Innovation Center of Post-Harvest Key Technology and Quality Safety of Fruits and Vegetables, Jiangxi Agricultural University, Nanchang 330045, China, [email protected].

PMID: 30046248 PMCID: PMC6054273 DOI: 10.2147/DMSO.S166728

Abstract

BACKGROUND: To investigate hypoglycemic activity and elucidate the active composition of the fruit blueberry (

METHODS: Methanol extracts of blueberry (MEB) were separated using a D101 macroporous resin column to yield quinic acid derivative (Fr.1)- and flavonoid (Fr.2)-rich fractions. The effects of the blueberry extracts on mRNA expression of GLUT-2 (glucose transporter type 2) and PPARγ (peroxisome proliferator-activated receptor-γ), as well as on the activities of PPRE (peroxisome proliferator response element) and NF-κB were analyzed in LO2 normal liver cells. Real-time PCR was used to detect the expression of GLUT-2, PPARγ, TNF-α, IL-1β, and IL-6 mRNA. The PPRE and NF-κB activities were detected by a luciferase reporter assay. Western blotting was used to detect the levels of PPARγ, GLUT-2, and p65. The active compositions were isolated using various chromatography columns, and were analyzed by NMR.

RESULTS: mRNA and protein expression of GLUT-2 and PPARγ were significantly increased upon treatment with 400 μg/mL extracts of blueberry (

CONCLUSION: Blueberry may have hypoglycemic activity that functions through synergistic effects with caffeoylquinic acid derivatives and quercetin glycosides.

Keywords: blueberry; caffeoylquinic acid derivatives; fla-vonoids; hypoglycemic activity; liver cells; synergistic effect

Conflict of interest statement

Disclosure The authors declare no conflicts of interest in this work.

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