Display options
Cite
Hodgetts J. Rapid Sample Preparation and LAMP for Phytoplasma Detection. Methods Mol Biol. 2019;1875:187-201doi: 10.1007/978-1-4939-8837-2_15.
Hodgetts, J. (2019). Rapid Sample Preparation and LAMP for Phytoplasma Detection. Methods in molecular biology (Clifton, N.J.), 1875187-201. https://doi.org/10.1007/978-1-4939-8837-2_15
Hodgetts, Jennifer. "Rapid Sample Preparation and LAMP for Phytoplasma Detection." Methods in molecular biology (Clifton, N.J.) vol. 1875 (2019): 187-201. doi: https://doi.org/10.1007/978-1-4939-8837-2_15
Hodgetts J. Rapid Sample Preparation and LAMP for Phytoplasma Detection. Methods Mol Biol. 2019;1875:187-201. doi: 10.1007/978-1-4939-8837-2_15. PMID: 30362005.
Copy Download .nbib Format: NLM
Share it on

Methods Mol Biol. 2019;1875:187-201. doi: 10.1007/978-1-4939-8837-2_15.

Rapid Sample Preparation and LAMP for Phytoplasma Detection.

Methods in molecular biology (Clifton, N.J.)

Jennifer Hodgetts

Affiliations

  1. Fera, The National Agri-Food Innovation Campus, Sand Hutton, York, UK. [email protected].

PMID: 30362005 DOI: 10.1007/978-1-4939-8837-2_15

Abstract

Loop-mediated isothermal AMPlification (LAMP) allows the rapid detection of pathogens by polymerase-mediated amplification of target nucleic acid sequences at a single incubation temperature. LAMP can be combined with very simple sample preparation/crude DNA extraction protocols, allowing the method to be used away from the laboratory for in-field detection. Equally, these benefits can also be leveraged to provide a rapid method suited to high-throughput diagnostic laboratories. In this chapter we described a crude DNA extraction protocol suitable for use in the field and provide a protocol for real-time detection using LAMP.

Keywords: Crude DNA extraction; In-field detection; Loop-mediated isothermal AMPlification (LAMP); PEG; Rapid testing

Substances

MeSH terms

Publication Types