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Gleich A, Kaiser B, Honscha W, et al. Evaluation of the hepatocyte-derived cell line BFH12 as an in vitro model for bovine biotransformation. Cytotechnology. 2019;71(1):231-244doi: 10.1007/s10616-018-0279-4.
Gleich, A., Kaiser, B., Honscha, W., Fuhrmann, H., & Schoeniger, A. (2019). Evaluation of the hepatocyte-derived cell line BFH12 as an in vitro model for bovine biotransformation. Cytotechnology, 71(1), 231-244. https://doi.org/10.1007/s10616-018-0279-4
Gleich, Alexander, et al. "Evaluation of the hepatocyte-derived cell line BFH12 as an in vitro model for bovine biotransformation." Cytotechnology vol. 71,1 (2019): 231-244. doi: https://doi.org/10.1007/s10616-018-0279-4
Gleich A, Kaiser B, Honscha W, Fuhrmann H, Schoeniger A. Evaluation of the hepatocyte-derived cell line BFH12 as an in vitro model for bovine biotransformation. Cytotechnology. 2019 Feb;71(1):231-244. doi: 10.1007/s10616-018-0279-4. Epub 2019 Jan 08. PMID: 30617848; PMCID: PMC6368520.
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Cytotechnology. 2019 Feb;71(1):231-244. doi: 10.1007/s10616-018-0279-4. Epub 2019 Jan 08.

Evaluation of the hepatocyte-derived cell line BFH12 as an in vitro model for bovine biotransformation.

Cytotechnology

Alexander Gleich, Bastian Kaiser, Walther Honscha, Herbert Fuhrmann, Axel Schoeniger

Affiliations

  1. Institute of Biochemistry, University of Leipzig, An den Tierkliniken 1, 04103, Leipzig, Germany.
  2. Institute of Veterinary Physiology, University of Leipzig, An den Tierkliniken 7, 04103, Leipzig, Germany.
  3. Institute of Veterinary Pharmacology and Toxicology, University of Leipzig, An den Tierkliniken 15, 04103, Leipzig, Germany.
  4. Institute of Biochemistry, University of Leipzig, An den Tierkliniken 1, 04103, Leipzig, Germany. [email protected].

PMID: 30617848 PMCID: PMC6368520 DOI: 10.1007/s10616-018-0279-4

Abstract

The knowledge of drug metabolising enzymes (DMEs) in cattle is rather limited. The capability of the bovine foetal hepatocyte-derived cell line BFH12 to serve as model for biotransformation was evaluated. Gene expression analysis of DMEs was performed by reverse transcription PCR (RT-PCR). The presence of efflux transporters was visualised by immunocytochemistry, and functional induction of cytochrome P450 (CYP) 1A was assessed by the ethoxyresorufin-O-deethylase (EROD) assay. The production of bile acids was measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). RT-PCR revealed the expression of cytochromes 1A1, 1A2, 3A4 and phase II enzymes UGT1A1, UGT1A6 and GSTM1. Immunofluorescence demonstrated efflux transporters ABCG2 and ABCC1. The EROD assay revealed a dose-dependent CYP1A induction after treatment with benzo[a]pyrene (BP). LC-MS/MS analysis of cell culture supernatants showed the production of bile acids including taurocholic acid, tauro-chenodeoxycholic acid, taurodeoxycholic acid and taurolithocholic acid. The results strongly suggest the applicability of the cell line BFH12 for subsequent experiments in the emerging field of bovine biotransformation.

Keywords: Bile acid; Biotransformation; Bovine hepatocyte; Cattle; Cytochrome P450; In vitro model

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