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Wei XY, Li JH, Wang L, et al. Low-voltage electrical cell lysis using a microfluidic device. Biomed Microdevices. 2019;21(1):22doi: 10.1007/s10544-019-0369-x.
Wei, X. Y., Li, J. H., Wang, L., & Yang, F. (2019). Low-voltage electrical cell lysis using a microfluidic device. Biomedical microdevices, 21(1), 22. https://doi.org/10.1007/s10544-019-0369-x
Wei, Xiao-Yu, et al. "Low-voltage electrical cell lysis using a microfluidic device." Biomedical microdevices vol. 21,1 (2019): 22. doi: https://doi.org/10.1007/s10544-019-0369-x
Wei XY, Li JH, Wang L, Yang F. Low-voltage electrical cell lysis using a microfluidic device. Biomed Microdevices. 2019 Feb 21;21(1):22. doi: 10.1007/s10544-019-0369-x. PMID: 30790126.
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Biomed Microdevices. 2019 Feb 21;21(1):22. doi: 10.1007/s10544-019-0369-x.

Low-voltage electrical cell lysis using a microfluidic device.

Biomedical microdevices

Xiao-Yu Wei, Jin-Hua Li, Lei Wang, Fang Yang

Affiliations

  1. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, 130012, China.
  2. Changchun Experimental School of the Affiliated High School of Jilin University, Jilin University, Changchun, 130021, China.
  3. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, 130012, China. [email protected].

PMID: 30790126 DOI: 10.1007/s10544-019-0369-x

Abstract

Cell lysis, where cellular material is released, is the basis for the separation and purification of cell contents, biochemical analysis, and other related experiments. It is also a key step in molecular, real-time, and cancer diagnoses as well as in the drug screening of pathogens. The current methods of lysing cells have several limitations, such as damage to the activity of cellular components, the need for a large number of cell samples, time-consuming processes, and the danger of high voltage. Therefore, a simple, fast, and efficient method for the manipulation of micro-volume cells or for single cell lysis is significant for further scientific research and practical application. In this study, a new low-voltage controllable method for cell lysis was established, and a corresponding microfluidic chip was developed. Simple, efficient and rapid micro-volume cells and single cell lysis were successfully achieved under a low-voltage alternating current with a voltage of 16 V

Keywords: Alternating current; Electrical cell lysis; Low voltage; Microfluidics

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