Display options
Cite
Bautista WW, Osiowy C, Klein J, et al. Hepatitis B Virus Infection of Normal Hepatic Stem/Progenitor Cells. J Clin Exp Hepatol. 2018;9(1):34-42doi: 10.1016/j.jceh.2018.02.002.
Bautista, W. W., Osiowy, C., Klein, J., & Minuk, G. Y. (2019). Hepatitis B Virus Infection of Normal Hepatic Stem/Progenitor Cells. Journal of clinical and experimental hepatology, 9(1), 34-42. https://doi.org/10.1016/j.jceh.2018.02.002
Bautista, Wendy W, et al. "Hepatitis B Virus Infection of Normal Hepatic Stem/Progenitor Cells." Journal of clinical and experimental hepatology vol. 9,1 (2019): 34-42. doi: https://doi.org/10.1016/j.jceh.2018.02.002
Bautista WW, Osiowy C, Klein J, Minuk GY. Hepatitis B Virus Infection of Normal Hepatic Stem/Progenitor Cells. J Clin Exp Hepatol. 2019 Jan-Feb;9(1):34-42. doi: 10.1016/j.jceh.2018.02.002. Epub 2018 Feb 16. PMID: 30765937; PMCID: PMC6363946.
Copy Download .nbib Format: NLM
Share it on

J Clin Exp Hepatol. 2019 Jan-Feb;9(1):34-42. doi: 10.1016/j.jceh.2018.02.002. Epub 2018 Feb 16.

Hepatitis B Virus Infection of Normal Hepatic Stem/Progenitor Cells.

Journal of clinical and experimental hepatology

Wendy W Bautista, Carla Osiowy, Julianne Klein, Gerald Y Minuk

Affiliations

  1. Section of Hepatology, Department of Medicine, University of Manitoba, Winnipeg, Canada.
  2. National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Canada.
  3. Diagnostic Services Manitoba, Department of Pathology, St. Boniface General Hospital, University of Manitoba, Winnipeg, Canada.

PMID: 30765937 PMCID: PMC6363946 DOI: 10.1016/j.jceh.2018.02.002

Abstract

BACKGROUND/AIMS: Whether the hepatitis B virus (HBV) infects normal hepatic stem/progenitor cells (NSCs) and if so, whether such infections play a role in the pathogenesis of HBV-induced chronic liver disease (CLD) and/or hepatocellular carcinoma (HCC) remains to be determined. The objectives of this study were to determine whether HBV infects NSCs and whether such infections alter NSC activity in a manner likely to contribute to the development of CLD and/or HCC.

METHODS: Liver biopsies from five hepatitis B surface antigen (HBsAg) positive patients were co-stained for HBcAg and HBx and the stem cell markers EpCAM, Oct-4 and Nanog. In addition, primary NSCs derived from healthy human livers were exposed to HBV contaminated serum

RESULTS: Markers of HBV infection were present within the NSC population of all five biopsy specimens.

CONCLUSIONS: Although the results of this study need to be confirmed, they suggest that HBV infects human NSCs but in the short term, do not alter those NSC features or activities associated with CLD and/or HCC.

Keywords: IU, international units; Neg, negative; SN, supernatant; UND, undetected; chronic hepatitis B; d, days; hepatic progenitor cells; hepatic stem cells; hepatitis B virus; hepatocellular carcinoma; ml, milliliters

References

  1. J Hepatol. 1999 Mar;30(3):492-7 - PubMed
  2. Semin Liver Dis. 1999;19(2):157-69 - PubMed
  3. Carcinogenesis. 2003 Jul;24(7):1167-76 - PubMed
  4. J Viral Hepat. 2005 Jul;12(4):364-72 - PubMed
  5. Gastroenterology. 2005 Jul;129(1):234-45 - PubMed
  6. Drugs Today (Barc). 2007 Jun;43(6):379-94 - PubMed
  7. Adv Biochem Eng Biotechnol. 2007;106:41-73 - PubMed
  8. Cell Biol Int. 2009 Apr;33(4):493-500 - PubMed
  9. Hepatogastroenterology. 2009 Jul-Aug;56(93):964-7 - PubMed
  10. Clin Liver Dis. 2010 Nov;14(4):705-18 - PubMed
  11. World J Hepatol. 2010 Mar 27;2(3):114-26 - PubMed
  12. Cancer Res. 2011 May 15;71(10):3701-8 - PubMed
  13. Virol J. 2011 Oct 31;8:486 - PubMed
  14. Gut. 2012 Dec;61(12):1754-64 - PubMed
  15. Methods Mol Biol. 2012;826:25-32 - PubMed
  16. Arch Pharm Res. 2012 Feb;35(2):213-21 - PubMed
  17. Arch Pharm Res. 2012 Feb;35(2):261-9 - PubMed
  18. Hepatology. 2013 Jan;57(1):59-69 - PubMed
  19. J Hepatol. 2013 Oct;59(4):746-52 - PubMed
  20. Gastroenterology. 2014 Apr;146(4):1070-83 - PubMed
  21. Can J Physiol Pharmacol. 2014 Jan;92(1):85-91 - PubMed
  22. Int J Mol Sci. 2014 Apr 10;15(4):6096-110 - PubMed
  23. Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):12193-8 - PubMed
  24. Cell Immunol. 2014 Nov-Dec;292(1-2):94-101 - PubMed
  25. Med Hypotheses. 2016 Jun;91:24-27 - PubMed
  26. Can J Infect Dis Med Microbiol. 2016;2016:8931591 - PubMed
  27. Am J Physiol. 1987 May;252(5 Pt 1):G642-7 - PubMed
  28. N Engl J Med. 1997 Dec 11;337(24):1733-45 - PubMed

Publication Types