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Gutierrez J, Guimaraes AO, Lewin-Koh N, et al. Sustained Circulating Bacterial Deoxyribonucleic Acid Is Associated With Complicated . Open Forum Infect Dis. 2019;6(4):ofz090doi: 10.1093/ofid/ofz090.
Gutierrez, J., Guimaraes, A. O., Lewin-Koh, N., Berhanu, A., Xu, M., Cao, Y., Kim, J., Yan, D., Chang, J. K., Dinoso, J. B., Koss, C. A., Clemenzi-Allen, A., Chambers, H. F., Peck, M. C., Baruch, A., & Rosenberger, C. M. (2019). Sustained Circulating Bacterial Deoxyribonucleic Acid Is Associated With Complicated . Open forum infectious diseases, 6(4), ofz090. https://doi.org/10.1093/ofid/ofz090
Gutierrez, Johnny, et al. "Sustained Circulating Bacterial Deoxyribonucleic Acid Is Associated With Complicated ." Open forum infectious diseases vol. 6,4 (2019): ofz090. doi: https://doi.org/10.1093/ofid/ofz090
Gutierrez J, Guimaraes AO, Lewin-Koh N, Berhanu A, Xu M, Cao Y, Kim J, Yan D, Chang JK, Dinoso JB, Koss CA, Clemenzi-Allen A, Chambers HF, Peck MC, Baruch A, Rosenberger CM. Sustained Circulating Bacterial Deoxyribonucleic Acid Is Associated With Complicated . Open Forum Infect Dis. 2019 Feb 26;6(4):ofz090. doi: 10.1093/ofid/ofz090. eCollection 2019 Apr. PMID: 31024970; PMCID: PMC6475589.
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Open Forum Infect Dis. 2019 Feb 26;6(4):ofz090. doi: 10.1093/ofid/ofz090. eCollection 2019 Apr.

Sustained Circulating Bacterial Deoxyribonucleic Acid Is Associated With Complicated .

Open forum infectious diseases

Johnny Gutierrez, Alessander O Guimaraes, Nicholas Lewin-Koh, Aklile Berhanu, Min Xu, Yi Cao, Janice Kim, Donghong Yan, Joanna K Chang, Jason B Dinoso, Catherine A Koss, Angelo Clemenzi-Allen, Henry F Chambers, Melicent C Peck, Amos Baruch, Carrie M Rosenberger

Affiliations

  1. Genentech, Inc., South San Francisco, California.
  2. University of California San Francisco, California.

PMID: 31024970 PMCID: PMC6475589 DOI: 10.1093/ofid/ofz090

Abstract

METHODS: A highly sensitive quantitative polymerase chain reaction was used to measure whole blood SA-DNA and plasma-derived SA cell-free DNA (SA-cfDNA) in a set of longitudinal samples from 73 patients with confirmed SA bacteremia and correlated with clinical features.

RESULTS: Blood SA-DNA was detected for longer than the duration of positive blood cultures. Longer duration of circulating bacterial DNA was observed in complicated SA bacteremia infections, such as endocarditis and osteoarticular infections, compared with uncomplicated bloodstream infections. In contrast, traditional blood cultures demonstrated similar time to clearance regardless of foci of infection. Plasma-derived SA-cfDNA showed concordance with blood SA-DNA levels. Baseline levels of SA-DNA were higher in patients presenting with greater clinical severity and complicated bacteremia.

CONCLUSIONS: Prolonged levels of circulating SA-DNA in patients with complicated tissue reservoirs after clearance of blood cultures observed in this single-center study should be validated in additional cohorts to assess the potential utility for monitoring clearance of infection in patients with SA bacteremia.

Keywords: Staphylococcus aureus; bacteremia; cell-free DNA; circulating DNA; prognostic biomarkers

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