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Trimidal SG, Benjamin R, Bae JE, et al. Can Designer Indels Be Tailored by Gene Editing?: Can Indels Be Customized?. Bioessays. 2019;41(12):e1900126doi: 10.1002/bies.201900126.
Trimidal, S. G., Benjamin, R., Bae, J. E., Han, M. V., Kong, E., Singer, A., Williams, T. S., Yang, B., & Schiller, M. R. (2019). Can Designer Indels Be Tailored by Gene Editing?: Can Indels Be Customized?. BioEssays : news and reviews in molecular, cellular and developmental biology, 41(12), e1900126. https://doi.org/10.1002/bies.201900126
Trimidal, Sara G, et al. "Can Designer Indels Be Tailored by Gene Editing?: Can Indels Be Customized?." BioEssays : news and reviews in molecular, cellular and developmental biology vol. 41,12 (2019): e1900126. doi: https://doi.org/10.1002/bies.201900126
Trimidal SG, Benjamin R, Bae JE, Han MV, Kong E, Singer A, Williams TS, Yang B, Schiller MR. Can Designer Indels Be Tailored by Gene Editing?: Can Indels Be Customized?. Bioessays. 2019 Dec;41(12):e1900126. doi: 10.1002/bies.201900126. Epub 2019 Nov 06. PMID: 31693213; PMCID: PMC7202862.
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Bioessays. 2019 Dec;41(12):e1900126. doi: 10.1002/bies.201900126. Epub 2019 Nov 06.

Can Designer Indels Be Tailored by Gene Editing?: Can Indels Be Customized?.

BioEssays : news and reviews in molecular, cellular and developmental biology

Sara G Trimidal, Ronald Benjamin, Ji Eun Bae, Mira V Han, Elizabeth Kong, Aaron Singer, Tyler S Williams, Bing Yang, Martin R Schiller

Affiliations

  1. School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV, 89154, USA.
  2. Nevada Institute of Personalized Medicine, University of Nevada Las Vegas, Las Vegas, NV, 89154, USA.
  3. Donald Danforth Plant Science Center, St. Louis, MO, 63132, USA.

PMID: 31693213 PMCID: PMC7202862 DOI: 10.1002/bies.201900126

Abstract

Genome editing with engineered nucleases (GEENs) introduce site-specific DNA double-strand breaks (DSBs) and repairs DSBs via nonhomologous end-joining (NHEJ) pathways that eventually create indels (insertions/deletions) in a genome. Whether the features of indels resulting from gene editing could be customized is asked. A review of the literature reveals how gene editing technologies via NHEJ pathways impact gene editing. The survey consolidates a body of literature that suggests that the type (insertion, deletion, and complex) and the approximate length of indel edits can be somewhat customized with different GEENs and by manipulating the expression of key NHEJ genes. Structural data suggest that binding of GEENs to DNA may interfere with binding of key components of DNA repair complexes, favoring either classical- or alternative-NHEJ. The hypotheses have some limitations, but if validated, will enable scientists to better control indel makeup, holding promise for basic science and clinical applications of gene editing. Also see the video abstract here https://youtu.be/vTkJtUsLi3w.

© 2019 WILEY Periodicals, Inc.

Keywords: CRISPR/Cas9; TALEN; double strand break; gene editing; meganuclease; non-homologous end joining; zinc finger nuclease

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