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Morgan K, Gamal W, Samuel K, et al. Application of Impedance-Based Techniques in Hepatology Research. J Clin Med. 2019;9(1)doi: 10.3390/jcm9010050.
Morgan, K., Gamal, W., Samuel, K., Morley, S. D., Hayes, P. C., Bagnaninchi, P., & Plevris, J. N. (2019). Application of Impedance-Based Techniques in Hepatology Research. Journal of clinical medicine, 9(1), . https://doi.org/10.3390/jcm9010050
Morgan, Katie, et al. "Application of Impedance-Based Techniques in Hepatology Research." Journal of clinical medicine vol. 9,1 (2019). doi: https://doi.org/10.3390/jcm9010050
Morgan K, Gamal W, Samuel K, Morley SD, Hayes PC, Bagnaninchi P, Plevris JN. Application of Impedance-Based Techniques in Hepatology Research. J Clin Med. 2019 Dec 24;9(1). doi: 10.3390/jcm9010050. PMID: 31878354; PMCID: PMC7019217.
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J Clin Med. 2019 Dec 24;9(1). doi: 10.3390/jcm9010050.

Application of Impedance-Based Techniques in Hepatology Research.

Journal of clinical medicine

Katie Morgan, Wesam Gamal, Kay Samuel, Steven D Morley, Peter C Hayes, Pierre Bagnaninchi, John N Plevris

Affiliations

  1. The University of Edinburgh Hepatology Laboratory, Division of Heath Sciences, University of Edinburgh Medical School, Chancellor's Building, Edinburgh BioQuarter, 49 Little France Crescent, EH16 4SB Edinburgh, UK.
  2. James Nasmyth Building, Institute of Mechanical, Process and Energy Engineering, Heriot-Watt University School of Engineering and Physical Sciences, EH14 4AS Edinburgh, UK.
  3. The Jack Copland Centre, Advanced Therapeutics, Scottish National Blood Transfusion Service, 52 Research Avenue North, EH14 4BE Edinburgh, UK.
  4. MRC Centre for Regenerative Medicine 5 Little France Drive, EH16 4UU Edinburgh, UK.

PMID: 31878354 PMCID: PMC7019217 DOI: 10.3390/jcm9010050

Abstract

There are a variety of end-point assays and techniques available to monitor hepatic cell cultures and study toxicity within in vitro models. These commonly focus on one aspect of cell metabolism and are often destructive to cells. Impedance-based cellular assays (IBCAs) assess biological functions of cell populations in real-time by measuring electrical impedance, which is the resistance to alternating current caused by the dielectric properties of proliferating of cells. While the uses of IBCA have been widely reported for a number of tissues, specific uses in the study of hepatic cell cultures have not been reported to date. IBCA monitors cellular behaviour throughout experimentation non-invasively without labelling or damage to cell cultures. The data extrapolated from IBCA can be correlated to biological events happening within the cell and therefore may inform drug toxicity studies or other applications within hepatic research. Because tight junctions comprise the blood/biliary barrier in hepatocytes, there are major consequences when these junctions are disrupted, as many pathologies centre around the bile canaliculi and flow of bile out of the liver. The application of IBCA in hepatology provides a unique opportunity to assess cellular polarity and patency of tight junctions, vital to maintaining normal hepatic function. Here, we describe how IBCAs have been applied to measuring the effect of viral infection, drug toxicity /IC50, cholangiopathies, cancer metastasis and monitoring of the gut-liver axis. We also highlight key areas of research where IBCAs could be used in future applications within the field of hepatology.

Keywords: ECIS; TEER; hepatology; impedance based cellular assay; liver; xCELLigence

Conflict of interest statement

The authors have no conflict of interest to declare with respect to this manuscript.

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