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Liu J, Deng JL, Tian Y. Transcriptome sequencing of the apricot (Prunus armeniaca L.) and identification of differentially expressed genes involved in drought stress. Phytochemistry. 2020;171:112226doi: 10.1016/j.phytochem.2019.112226.
Liu, J., Deng, J. L., & Tian, Y. (2020). Transcriptome sequencing of the apricot (Prunus armeniaca L.) and identification of differentially expressed genes involved in drought stress. Phytochemistry, 171112226. https://doi.org/10.1016/j.phytochem.2019.112226
Liu, Jia, et al. "Transcriptome sequencing of the apricot (Prunus armeniaca L.) and identification of differentially expressed genes involved in drought stress." Phytochemistry vol. 171 (2020): 112226. doi: https://doi.org/10.1016/j.phytochem.2019.112226
Liu J, Deng JL, Tian Y. Transcriptome sequencing of the apricot (Prunus armeniaca L.) and identification of differentially expressed genes involved in drought stress. Phytochemistry. 2020 Mar;171:112226. doi: 10.1016/j.phytochem.2019.112226. Epub 2020 Jan 08. PMID: 31923721.
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Phytochemistry. 2020 Mar;171:112226. doi: 10.1016/j.phytochem.2019.112226. Epub 2020 Jan 08.

Transcriptome sequencing of the apricot (Prunus armeniaca L.) and identification of differentially expressed genes involved in drought stress.

Phytochemistry

Jia Liu, Jia Lin Deng, Yun Tian

Affiliations

  1. Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, 610066, PR China; Southwestern Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement, Ministry of Agriculture, Chengdu, Sichuan, 610066, PR China.
  2. Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, 610066, PR China; Southwestern Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement, Ministry of Agriculture, Chengdu, Sichuan, 610066, PR China. Electronic address: [email protected].

PMID: 31923721 DOI: 10.1016/j.phytochem.2019.112226

Abstract

Apricot (Prunus armeniaca L.) is an important fruit crop that is widely planted throughout the world. But drought affects both yield and quality of apricot. In order to study the effects of long-term drought on the molecular and physiological mechanisms of apricot, we used transcriptome sequencing and measured physiological indices. First, 322 million high-quality clean reads were obtained, and 74,892 unigenes were generated for the transcriptome. Among the assembled unigenes, 18,671 simple sequence repeats (SSRs) and 5581 differentially expressed genes (DEGs) were identified. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the DEGs revealed that starch and sucrose metabolism, plant-pathogen interaction and plant hormone signal transduction pathways are enriched. Additionally, we used quantitative real-time PCR (qRT-PCR) to confirm the RNA-seq results with 11 drought-related DEGs. Second, through the physiological analysis of apricot leaves under constant drought stress, and the results show the internal microstructure of apricot leaves changed to withstand drought stress. At the same time, plants exposed to long-term drought stress showed higher degree of membrane damage, which reduced photosynthesis in the damaged leaves. Our findings enrich the genome resources for apricot and refine our understanding of the molecular and physiological mechanisms of drought response in this fruit crop, providing insights into drought adaptation of the apricot.

Copyright © 2020 Elsevier Ltd. All rights reserved.

Keywords: Apricot; Differentially expressed genes; Drought stress; Physiological analysis; Prunus armeniaca; Transcriptome sequencing

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