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Cook L, Munier CML, Seddiki N, et al. Circulating gluten-specific, but not CMV-specific, CD39. Clin Transl Immunology. 2020;9(1):e1096doi: 10.1002/cti2.1096.
Cook, L., Munier, C. M. L., Seddiki, N., Hardy, M. Y., Anderson, R. P., Zaunders, J., Tye-Din, J. A., Kelleher, A. D., & van Bockel, D. (2020). Circulating gluten-specific, but not CMV-specific, CD39. Clinical & translational immunology, 9(1), e1096. https://doi.org/10.1002/cti2.1096
Cook, Laura, et al. "Circulating gluten-specific, but not CMV-specific, CD39." Clinical & translational immunology vol. 9,1 (2020): e1096. doi: https://doi.org/10.1002/cti2.1096
Cook L, Munier CML, Seddiki N, Hardy MY, Anderson RP, Zaunders J, Tye-Din JA, Kelleher AD, van Bockel D. Circulating gluten-specific, but not CMV-specific, CD39. Clin Transl Immunology. 2020 Jan 12;9(1):e1096. doi: 10.1002/cti2.1096. eCollection 2020. PMID: 31956412; PMCID: PMC6955237.
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Clin Transl Immunology. 2020 Jan 12;9(1):e1096. doi: 10.1002/cti2.1096. eCollection 2020.

Circulating gluten-specific, but not CMV-specific, CD39.

Clinical & translational immunology

Laura Cook, C Mee Ling Munier, Nabila Seddiki, Melinda Y Hardy, Robert P Anderson, John Zaunders, Jason A Tye-Din, Anthony D Kelleher, David van Bockel

Affiliations

  1. Immunovirology and Pathogenesis Program The Kirby Institute UNSW Australia Sydney NSW Australia.
  2. St Vincent's Centre for Applied Medical Research St Vincent's Hospital Sydney NSW Australia.
  3. Present address: Department of Medicine and BC Children's Hospital Research Institute University of British Columbia Vancouver Canada.
  4. Present address: INSERM U955 and Université Paris-Est Créteil (UPEC)/Vaccine Research Institute Créteil France.
  5. Immunology Division Walter and Eliza Hall Institute Parkville VIC Australia.
  6. Department of Medical Biology The University of Melbourne Parkville VIC Australia.
  7. ImmusanT, Inc. Cambridge MA USA.
  8. Department of Gastroenterology The Royal Melbourne Hospital Parkville VIC Australia.

PMID: 31956412 PMCID: PMC6955237 DOI: 10.1002/cti2.1096

Abstract

OBJECTIVES: Understanding the T cell receptor (TCR) repertoire of regulatory CD4

METHODS: We used the OX40 assay to isolate antigen-specific Tregs by induced surface co-expression of CD25, OX40 and CD39. RACE PCR amplification and Sanger sequencing of the TCR β chain were used to analyse repertoire diversity.

RESULTS: We found that, following oral gluten challenge, circulating gluten-specific CD39

DISCUSSION: These data indicate that a biased TCR repertoire is not inherent to CD39

CONCLUSION: This is the first assessment of the TCR repertoire within circulating human Tregs specific for foreign antigen. These data enhance our understanding of antigen-specific CD4

© 2020 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology Inc.

Keywords: CD4+ T cells; CMV; TCR repertoire; coeliac disease; gluten; regulatory T cells

Conflict of interest statement

RPA and JT‐D are co‐inventors of patents pertaining to the use of gluten peptides in therapeutics, diagnostics and non‐toxic gluten; both are shareholders of Nexpep Pty Ltd and RPA also of ImmusanT, I

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